| CCKB-R mRNA在幼龄厌食大鼠结肠平滑肌细胞中的表达 |
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| 引用本文： | 赵琼,廖敏,丁维俊,李秀亮,瞿佳.CCKB-R mRNA在幼龄厌食大鼠结肠平滑肌细胞中的表达[J].时珍国医国药,2009,20(7). |
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| 作者姓名： | 赵琼 廖敏 丁维俊 李秀亮 瞿佳 |
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| 作者单位： | 成都中医药大学,四川,成都,611137 |
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| 基金项目： | 四川省教育厅青年基金 |
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| 摘 要： | 目的 探讨胆囊收缩素受体CCKB-R mRNA在幼龄厌食大鼠结肠平滑肌细胞中的表达及加减益胃汤对其表达的影响.方法 借助特制饲料喂养幼龄厌食大鼠模型,采用RT-PCR技术检测CCKB-R在幼龄厌食大鼠结肠平滑肌细胞中的表达,并观察加减益胃汤对该模型CCKB-R表达的影响.结果 正常大鼠结肠平滑肌细胞中CCKB-R有低水平表达,特制饲料喂养造模后其表达水平异常增高,与空白对照组比较有极显著的统计学意义(P＜0.001);加减益胃汤高、中、低剂量组均能显著抑制或下调CCKB-R的高表达,且有一定的量-效趋势.结论 CCKB-R基因高表达可能是厌食发生的主要病理环节之一,抑制或下调CCKB-R基因表达可能是加减益胃汤治疗厌食症的主要作用靶点之一.
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| 关 键 词： | 胆囊收缩素受体 厌食 |
Expression of CCKB-R mRNA in Colon Smooth Muscle Cells of the Anorexia Pedo-rats |
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| ZHAO Qiong,LIAO Min,DING Wei-jun,LI Xiu-liang,QU Jia.Expression of CCKB-R mRNA in Colon Smooth Muscle Cells of the Anorexia Pedo-rats[J].Lishizhen Medicine and Materia Medica Research,2009,20(7). |
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| Authors: | ZHAO Qiong LIAO Min DING Wei-jun LI Xiu-liang QU Jia |
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| Abstract: | Objective To investigate the expression of CCKB-R mRNA in colon smooth muscle cells of the anorexia pedo-rats,and the effect of modified Yi Wei Decoction on it.Methods The anorexia rat model was establisbed by feeding rats with specially prepared forage.The expression of CCKB-R mRNA in colon smooth muscle cells of the anorexia pedo-rats was examined by RT-PCR,and the effect of modified Yiwei decoction on it was observed.Results Compared with blank control,the expression of CCKB-R mRNA in anorexia rats was significantly increased (P＜0.001).Highdosage,middledosage or lowdosage of Yiwei decoction all could obviously decrease the expression of CCKB-R mRNA as compared with model group(P＜0.001).Conclusion The expression of CCKB-R mRNA can be obviously increased in colon smooth muscle cells of the anorexia pedo-rats,which is one of the mechanisms of anorexia.Modified Yiwei decoction can decrease the expression of CCKB-R mRNA in colon smooth muscle cells of the anorexia rats. |
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| Keywords: | CCKB-R Anorexia |
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