尿液中乙肝病毒DNA对HBV相关肾炎患者的诊断效果

目的 了解尿液中乙肝病毒DNA对HBV相关肾炎患者的诊断效果,为更好的诊断HBV相关肾炎(HBV-GN)提供依据.方法 选取于2013年10月～2014年10月在我院肾内科就诊的HBsAg阳性或血清HBV-DNA阳性者60例作为Ⅰ组、HBsAg阴性且清HBV-DNA阴性者60例作为Ⅱ组、同期在我院健康体检中心体检的健康者60例作为Ⅲ组.采用PCR方法检测各组研究对象尿中HBV DNA水平.结果 Ⅰ组中有38例(63.33％)患者尿HBV DNA呈阳性,Ⅱ组、Ⅲ组中无患者尿HBV DNA呈阳性.尿HBV DNA、血HBsAg和血HBeAg联合检查诊断HBV-GN的灵敏度和特异度均是最高的,其次为尿HBV DNA检测.结论 联合检测尿HBV DNA、血HBsAg和血HBeAg诊断HBV-GN效率比较高,尿HBV DNA可能作为HBV-GN的一种简单、无创、患者容易接受的辅助诊断指标.

Diagnostic values of hepatitis B virus DNA in urine for detection of HBV associated glomerulonephritis

Objective To investigate diagnostic values of Hepatitis B virus DNA in urine for HBV infection in patients with nephritis and to provide the basis for better diagnosis of HBV-GN patients.Methods From October 2013 to October 2014, 60 patients with serum HBsAg positive or HBV DNA positive nephrology were selected as group Ⅰ, 60 patients with serum HBsAg negative and HBV DNA negative nephrology as group Ⅱ and 60 healthy people as group Ⅲ.The PCR assay was used to detect HBV DNA levels in urine of patients in each group.Results For Group Ⅰ, HBV DNA positive rate was 63.33％ (38/60) and there was no positive case in group Ⅱ and group Ⅲ.The joint diagnosis pattern of urine HBV DNA , serum HB-sAg and HBeAg has highest sensitivity and specificity for diagnosis of HBV-GN patients.HBV DNA detection has higher sensitivity and specificity.Conclusions HBV DNA detectiong in urine may become a simple, noninvasive, diagnostic means.