脂联素rs266729多态性和肥胖在多囊卵巢综合征中交互作用研究

目的 探讨脂联素rs266729多态性与多囊卵巢综合征(PCOS)关联性及其与肥胖交互作用.方法 采用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)方法分析2014年2月至2015年8月住院的120例PCOS患者和同期140例体检正常对照者的rs266729基因型.非条件Logistic分析各基因型与发病易感性的关系以及与肥胖的交互作用.结果 携带C(CC/CT)基因个体患病风险较非C基因携带者(TT)风险明显增加1.55倍(OR=1.55,95%CI:1.41~3.71,P=0.00;校正OR=1.61,95%CI:1.51~4.01,P=0.00);非条件Logistic分析表明认携带CC/CT基因型肥胖个体PCOS罹患风险是携带TT基因非肥胖个体的3.88倍(OR=3.88,95%CI:2.72~5.76,P=0.00;RERI=2.51,95%CI:1.22~3.80;AP=0.54,95%CI:0.34~0.74;S=1.56,95%CI:1.12~2.83).结论 脂联素rs266729多态性增加多囊卵巢综合症罹患风险,且与肥胖存在多囊卵巢综合症发病中存在协同效应.

Interaction between adiponectin rs266729 polymorphisms and obesity in polycystic ovarian syndrome

Objective To study the relationship between rs266729 polymorphism in endothelial adiponectin and polycystic ovarian syndrome ( PCOS) and the interaction with obesity.Methods Genotypes of rs266729 of 120 hospitalized cases of PCOS and 140 controls selected from February 2014 to August 2015 were examined by polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) . The unconditional Logistic regression ( ULR) was performed to assess the correlation of genotypes with susceptibility and the interaction with obesity.Results The risk of developing PCOS for CC/CT genotype carriers increased by 1.55 times compared to TT ( OR=1.55, 95%CI:1.41-3.71,P=0.00; ORadjust =1.61,95%CI:1.51-4.01,P=0.00).ULR analysis showed that obesity-positive subjects with genotype CT/CC had 3.88 times of PCOS risk of obesity-negative subjects with genotype TT ( OR=3.88,95%CI:2.72-5.76,P=0.00) (RERI=2.51, 95%CI:1.22-3.80;API=0.54,95%CI:0.34-0.74;S=1.56,95%CI:1.12-2.83).Conclusion Adiponectin rs266729 polymorphism is associated with increased risk for PCOS, and its interaction with obesity has synergistic effects on risk of PCOS.