北京市吸毒人群HCV感染状况及基因特征分析

目的 分析北京市吸毒人群HCV感染情况及其HCV的病毒基因特征.方法 利用ELISA方法和Real-time PCR方法同时检测684名吸毒人群血清中HCV抗体和HCV RNA,确定该人群的HCV感染情况.对HCV抗体或RNA检测阳性的样本进行C/E1和NS5B基因区扩增并对扩增产物进行序列测定,分析吸毒人群HCV基因亚型构成.结果 吸毒人群HCV感染率为26.2％(179/684).142例样本的C/E1或NS5B基因区扩增成功,基因进化分析发现8种HCV基因亚型:1a、1b、2a、3a、3b、6a、6n和6u.结论 伴随着人口流动性的增加,目前北京市吸毒人群HCV流行情况复杂,具有多种基因亚型.

Subtypes and evolution characteristics of hepatitis C virus among drug users in Beijing

Objective The aim of this study was to characterize the current molecular epidemiology status of hepatitis C virus (HCV) infections and to evaluate the evolutionary patterns of HCV subtypes in Beijing,China.Methods The whole blood samples and behavioral data were collected from 684 drug users (DUs) in Beijing from 2010 to 2011.Samples were tested for HCV infection using both enzyme-linked immunosorbent assay (ELISA) and Real-time PCR.All viremic subjects were then sequenced by nested PCR over core/E1 and NSSB regions.Phylogenetic analysis was performed by Mega software.Results In total,179 subjects (26.17％) were tested positive for HCV by antibody or vRNA-based testing.The 142 HCV RNA-positive samples were sequenced.Eight HCV genotypes,including 1a,1b,2a,3a,3b,6a,6nand6u,were detected.Conclusions The current HCV epidemic in Beijing is complex,heavily affecting DUs,and involving multiple genotypes that likely spread from different regions in China with its large migrant population.