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Deep sequencing revealed genome-wide single-nucleotide polymorphism and plasmid content of Erwinia amylovora strains isolated in Middle Atlas,Morocco
Authors:Najat Hannou  Samuel Mondy  Sara Planamente  Mohieddine Moumni  Pablo Llop  María López  Charles Manceau  Marie-Anne Barny  Denis Faure
Institution:1. Université Moulay Ismail, Faculté des Sciences, Département de Biologie, Meknès, Morocco;2. Institut des Sciences du Végétal, CNRS, Avenue de la Terrasse, 91 198 Gif-sur-Yvette cedex, France;3. Instituto Valenciano de Investigaciones Agrarias, Carretera Moncada-Náquera km 4.5, 46113 Moncada, Valencia, Spain;4. Laboratoire de Pathologie Végétale, INRA, 42 rue Georges Morel, 49071 Beaucouzé, France;5. Laboratoire des Interactions Plantes-Pathogènes, AgroParisTech, 75231 Paris, France;1. Division of Pediatric, General and Thoracic Surgery, Cincinnati Children''s Hospital and the University of Cincinnati College of Medicine, Cincinnati, Ohio;2. Division of Pediatric Surgery, The Children''s Hospital Colorado, Aurora, Colorado;3. Division of Pediatric Surgery, Washington University School of Medicine, St. Louis, Missouri;4. Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, Tennessee;3. From the Department of Microbiology and;8. Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland,;4. INRA, UMR1319 Micalis, Jouy-en-Josas, France,;5. AgroParisTech, UMR Micalis, Jouy-en-Josas, France,;6. the Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824,;12. University of Copenhagen, Copenhagen, Denmark;1. Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, South Korea;2. Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, South Korea;3. Korea Clean Energy Center, Korea Institute of Science and Technology, Seoul 136-791, South Korea;4. Institute of Life Sciences and Resources & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 446-701, South Korea
Abstract:Erwinia amylovora causes economic losses that affect pear and apple production in Morocco. Here, we report comparative genomics of four Moroccan E. amylovora strains with the European strain CFBP1430 and North-American strain ATCC49946. Analysis of single nucleotide polymorphisms (SNPs) revealed genetic homogeneity of Moroccan's strains and their proximity to the European strain CFBP1430. Moreover, the collected sequences allowed the assembly of a 65 kpb plasmid, which is highly similar to the plasmid pEI70 harbored by several European E. amylovora isolates. This plasmid was found in 33% of the 40 E. amylovora strains collected from several host plants in 2009 and 2010 in Morocco.
Keywords:Genome  Plasmid  SNP  Virulence  Fire blight
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