新疆维吾尔族溃疡性结肠炎患者长链非编码RNA LINC00467表达特征与预后分析

目的探讨新疆维吾尔族溃疡性结肠炎(UC)患者长链非编码RNA(LncRNA)LINC00467表达特征与预后关系。方法回顾性分析2017年1月至2019年6月新疆医科大学第四附属医院收治的维吾尔族UC患者30例,其中活动期20例,缓解期10例。另选择2019年1月至2020年6月新疆医科大学第四附属医院维吾尔族健康人30例作为对照组。受试者均于结肠镜检查时,内镜直视下采用统一型号活检钳,取3块肠黏膜组织。采用荧光定量PCR测定LncRNA LINC00467表达。比较不同分期的UC患者LncRNA LINC00467表达;LncRNA LINC00467表达与UC患者临床特征的关系;比较不同预后的UC患者LncRNA LINC00467表达;采用Cox多因素分析影响预后危险因素。结果LncRNA LINC00467表达比较,活动期组(1.56±0.27)和缓解期组(0.89±0.21)高于对照组(0.34±0.08),活动期组(1.56±0.27)高于缓解期组(0.89±0.21),差异有统计学意义(P<0.05)。不同性别、年龄、病程和病变范围LncRNA LINC00467表达比较无统计学差异(P>0.05);LncRNA LINC00467表达比较,中度(1.30±0.25)和重度(1.67±0.24)高于(0.86±0.16)轻度,且重度(1.67±0.24)高于中度(1.30±0.25),差异有统计学意义(P<0.05)。随访末,预后良好21例,预后不良9例。预后不良组LncRNA LINC00467表达(2.42±0.48)高于预后良好组(0.63±0.12),差异有统计学意义(P<0.05)。经Cox多因素分析显示,疾病活动程度和LncRNA LINC00467表达为影响预后危险因素。结论新疆维吾尔族UC患者LncRNA LINC00467呈高表达,且随着病情加重及预后进展表达越高,及LncRNA LINC00467表达为影响危险因素。

Expression characteristics and prognosis of long non coding RNA linc00467 in Uygur patients with ulcerative colitis in Xinjiang

Objective To investigate the relationship between the expression of linc00467 and the prognosis of ulcerative colitis(UC).Methods The 30 patients with UC were selected from January 2018 to June 2019 in Fourth Affiliated Hospital of Xinjiang Medical University,including 20 cases in active stage and 10 cases in remission stage.Another 30 Uygur healthy people from January 2017 to June 2019 in Fourth Affiliated Hospital of Xinjiang Medical University were selected as control group.During colonoscopy,3 pieces of intestinal mucosa tissue were taken with the same type of biopsy forceps under direct endoscopy.The expression of lncrna linc00467 was detected by fluorescence quantitative PCR.The expression of LncRNA LINC00467 in different stages of UC patients was compared,the relationship between the expression of LncRNA LINC00467 and clinical characteristics was compared,and the expression of LncRNA LINC00467 in UC patients with different prognosis was compared.Cox multivariate analysis was used to analyze the prognostic risk factors.Results The expression of LncRNA LINC00467 in active phase group(1.56±0.27)and stable phase group(0.89±0.21)was higher than control group(0.34±0.08)(P<0.05);the expression of linc00467 in active phase group(1.56±0.27)was higher than stable phase group(0.89±0.21)(P<0.05).There was no significant difference in the expression of LncRNA LINC00467 among different gender,age,course of disease and lesion range(P>0.05);the expression of lincnrna linc00467 in moderate(1.30±0.25)and severe(1.67±0.24)was higher than(0.86±0.16)in mild,and severe(1.67±0.24)was higher than moderate(1.30±0.25)(P<0.05).At the end of follow-up,21 cases had good prognosis and 9 cases had poor prognosis.The expression of LncRNA LINC00467 in poor prognosis group(2.42±0.48)was higher than that in good prognosis group(0.63±0.12)(P<0.05).Cox multivariate analysis showed that disease activity and LncRNA LINC00467 expression were risk factors for prognosis.Conclusion LncRNA LINC00467 is highly expressed in Xinjiang Uygur patients with UC,and the higher the expression is with the aggravation of the disease and the progress of prognosis,and lncrna linc00467 expression is the risk factor.