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不同产地火麻仁的蛋白电泳分析
引用本文:吴发明,李敏,俸世洪,王兵,齐景梁.不同产地火麻仁的蛋白电泳分析[J].中国现代中药,2010,12(7):28-30.
作者姓名:吴发明  李敏  俸世洪  王兵  齐景梁
作者单位:成都中医药大学药学院,中药材标准化教育部重点实验室,四川,成都,611137
摘    要:目的:鉴定不同产地火麻仁,并探讨火麻仁的遗传多样性。方法:利用SDS-聚丙烯酰胺凝胶电泳技术,对不同外观性状的火麻仁进行蛋白电泳聚类分析。结果:火麻仁蛋白电泳图谱表现出可观察到的谱带18条,其中7条较明显的谱带为共有谱带,另外11条谱带具有多态性,反映了火麻仁的遗传多样性。结论:火麻仁蛋白稳定且具有丰富的遗传多样性,实验结果可为准确鉴定药用火麻仁提供辅助依据。

关 键 词:大麻  火麻仁  SDS-聚丙烯酰胺凝胶电泳
收稿时间:2010/2/28 0:00:00

Analysis of Cannabis Semen Properties and Protein Electrophoresis from Its Different Habitats
Wu Faming,Li Min,Feng Shihong,WangBing and Qi Jingliang.Analysis of Cannabis Semen Properties and Protein Electrophoresis from Its Different Habitats[J].Modern Chinese Medicine,2010,12(7):28-30.
Authors:Wu Faming  Li Min  Feng Shihong  WangBing and Qi Jingliang
Institution:Wu Faming, Li Min, Feng Shihong, WangBing ,Qi Jingliang;Wu Faming, Li Min, Feng Shihong, WangBing ,Qi Jingliang;Wu Faming, Li Min, Feng Shihong, WangBing ,Qi Jingliang;Wu Faming, Li Min, Feng Shihong, WangBing ,Qi Jingliang;Wu Faming, Li Min, Feng Shihong, WangBing ,Qi Jingliang
Abstract:Objective: To Identify the species and affinities of cannabis semen from different habitats, and investigate the relationships between heredity diversity and apparent property.Methods: Apply the SDS-polyacrylamide electrophoresis technology for the first time to take protein electrophoresis cluster analysis on the cannabis semen varied from apparent property. Results: Eighteen obvious spectral bands could be observed in the cannabis semen protein electrophoretogram, among which seven obvious and shared bands manifest inter-species consistency in cannabis semen. And the other eleven bands manifest the genetic polymorphism of cannabis semen. Conclusion: Protein of cannabis semen is stable and it has heredity diversity in abundance. The experimental result can provide theoretical support for the identification of medicinal Cannabis sativa L..
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