参芎化瘀胶囊对大鼠全脑缺血再灌注损伤及其海马CA1区生长相关蛋白43表达的影响

目的探讨参芎化瘀胶囊对大鼠全脑缺血再灌注损伤及海马CAl区生长相关蛋白43（growthassociatedprotein43，GAP43）表达的影响。方法100只成年SD雄性大鼠随机分为对照组、模型组和参芎化瘀胶囊每El1次组（中药A组），每日2次组（中药B组），每日3次组（中药C组），每组20只。采用改良的Pulsinelli四血管闭塞法制备全脑缺血再灌注动物模型。在1、3、7、14天4个时间点通过HE染色观察海马CAl区组织学改变，免疫组织化学法检测海马CAl区GAP43蛋白的表达同时测定行为学评分，第14天通过Westernblot法检测海马CAl区GAP43蛋白质含量。结果与对照组比较，模型组各时间点GAP43蛋白表达增高（P〈0．05），行为学评分升高（P〈0．05），变性神经元增加，存活神经元减少（P〈0．05）。与模型组比较，参芎化瘀胶囊各组GAP43蛋白表达均升高（P〈0．05），其中中药C组最明显（P〈0．01）；行为学评分显著降低（P〈0．05）；变性神经元数量减少，存活神经元数量增加（P〈0．05）；14天存活神经元数明显增多，其中以中药C组存活神经元数量最多、GAP43蛋白质含量最高，与中药A、B两组比较，差异有统计学意义（P〈0．01）。结论参芎化瘀胶囊对大鼠全脑缺血再灌注损伤具有保护作用，其机制可能与升高GAP43的表达有关。

Effect of Shenxiong Huayu Capsule on Cerebral Ischemia/Reperfusion Injury and the Expression of GAP43 in Hippocampal CA1 of Rats

Objective To explore the effect of Shenxiong Huayu Capsule （SHC） on the cerebral ischemia-reperfusion （IR） injury and the expression of growth associated protein 43 （GAP43） after total cerebral IR in the hippocampal CA1 region of rats, Methods Totally 100 male adult SD rats were ran- domly divided into five groups, i.e., the control group, the model group, the group A （by taking SHC once daily）, the group B （by taking SHC twice daily）, and the group C （by taking SHC thrice daily）, 20 in each group. The total IR model was prepared by improved Pulsinelli＇s 4-vessel occlusion method. Morpho- logical changes of the hippocampal CA1 region were observed by HE staining at day 1,3, 7, and 14. The expression of GAP43 in the hippocampal CA1 region was detected using immunohistochemical assay at day 1,3, 7, and 14. Meanwhile, the behavioral score was determined. The expression of GAP43 in the hippocampal CA1 region was detected using Western blot at day 14. Results Compared with the control group, the expression of GAP43 increased in the model group, the behavioral score was elevated, de- generated neurons increased, and survival neurons decreased in the model group （all P 〈0.05）. Com- pared with the model group, the expression of GAP-43 increased （with the most significant difference seen in the group C, P 〈0.01 ）, the behavioral score significantly decreased, degenerated neurons de- creased, and survival neurons increased in each HSC group （all P 〈0.05）. Survival neurons obviously increased at day 14, of which, most number of survival neurons and highest contents of GAP43 proteincould be seen in the group C, showing statistical difference when compared with those of the group A and the group B （P 〈0.01 ）. Conclusion SHC had protective effect on total cerebral IR in the hippocampal CA1, which might be associated with increased expression of GAP43.