胃癌组织中凋亡抑制因子Livin与半胱氨酸蛋白酶-9的表达及其相互关系

目的探讨胃癌组织中凋亡抑制因子livin和半胱氨酸蛋白酶-9(caspase-9)的表达情况及二者与胃癌生物学行为的关系,并探讨livin与caspase-9表达的相关性。方法采用免疫组化SP法检测正常胃黏膜组织和胃癌组织中livin和caspase-9的表达。结果胃癌组织livin和caspase-9的阳性表达率分别与正常胃黏膜组织的比较,差异有统计学意义(P<0.05)。livin阳性表达率在不同组织学分型、淋巴结转移、临床分期组间差异有统计学意义(P<0.05);caspase-9阳性表达率在淋巴结有无转移和不同临床分期组间差异有统计学意义(P<0.05)。livin表达与caspase-9表达呈负相关(r=-0.280,P<0.05)。结论livin和caspase-9参与了胃癌的发生、发展,二者表达呈负相关。

Expressions of Livin and Caspase-9 in Gastric Carcinoma Tissue and Their Relationship

Objective To study the expressions of apoptosis inhibitor livin and caspase-9 proteins in tissue of human gastric carcinoma,and to explore the relationship between their expressions and clinical pathology parameters,and correlation between livin expression and caspase-9 expression.Methods The expressions of livin and caspase-9 in gastric carcinoma tissues and normal gastric mucosa were detected by immunohistochemistry streptavidin peroxidase method.Results In gastric carcinoma tissues,the positive expressive rate of livin was significantly higher than that in normal gastric mucosa(P<0.05).and expression of livin was inversely correlated with the histological differentiation of the tumor,lymph node metastasis and clinical stage(P<0.05);the positive expressive rate of caspase-9 was significantly lower than that in normal gastric mucosa,and expression of caspase-9 was inversely correlated with the lymph node metastasis and clinical stage(P<0.05).There was a significant negative correlation between the expressions of livin and caspase-9 protein in gastric carcinoma tissues(r=-0.280,P<0.05).Conclusion Livin and caspase-9 are associated with occurrence and development of gastric cancer,and the expressions of the two are negatively correlated.