汉黄芩素对胶质瘤U251细胞Survivin mRNA及Caspase-3mRNA表达的影响研究

目的 探讨汉黄芩素对胶质瘤U251细胞Survivin mRNA及Caspase-3 mRNA表达的影响.方法 192株人胶质瘤U251细胞株分别用汉黄芩素（96株）、替莫唑胺（TMZ）（48株）处理,留取48株作为空白对照组,半定量RT-PCR法检测Survivin mRNA与Caspase-3 mRNA表达强度,并在3组之间进行对比分析.结果 空白对照组Survivin mRNA表达强度为（0.773±0.176） μM,显著高于汉黄芩素组的（0.382±0.087） μM及TMZ组的（0.408±0.092） μM （P ＜ 0.05）;Caspase-3 mRNA表达强度为（0.097±0.043） μM,显著低于汉黄芩素组的（0.454±0.213） μM及TMZ组的（0.432±0.187） μM（P ＜ 0.05）,汉黄芩组与TMZ组之间比较差异无统计学意义（P ＞ 0.05）.结论 汉黄芩素可通过下调Survivin mRNA的表达并上调Caspase-3 mRNA的表达起到有效诱导人胶质瘤U251细胞凋亡的作用.

Effects of wogonin on the expression of Survivin mRNA and Caspase-3 mRNA in human giloma cell line U251

Objective To discuss the effects of wogonin on the expression of Survivin mRNA and Caspase-3 mRNA in human giloma cell line U251. Methods One hundred and ninety-two human giloma cell line U251 were cultured with wogonin （96 strains） and TMZ （48 strains）, and other 48 strains were as blank control group, Survivin mRNA and Cas- pase-3 mRNA expression level were detected by semi-quantitative RT-PCR, and then Survivin mRNA and Caspase- 3 mRNA expression level were contrasted between the three groups. Results Survivin mRNA expression level in the blank control group was （0.773±0.176） μM, significantly higher than （0.382±0.087） μM in the wogonin group and （0.408±0.092） μM in the TMZ group （P 〈 0.05）, and Caspase-3 mRNA expression level was （0.097±0.043） μM, sig- nificantly lower than （0.454±0.213）μM in the wogonin group and （0.432±0.187） μM in the TMZ group （P 〈 0.05）, but there was no significantly difference in Survivin mRNA and Caspase-3 mRNA expression level between wogonin group and TMZ group （P 〉 0.05）. Conclusion Wogonin play a role in inducing apoptosis in glioma U251 cells through re- ducing the expression of Survivin mRNA and raising the expression of Caspase-3 protein expression.