流式细胞术检测急性杂合性白血病的临床分析

目的分析流式细胞术检测急性杂合性白血病的临床效果。方法对本院收治的急性杂合性白血病（HAL）患者、伴有淋系抗原表达型急性髓细胞白血病（Ly＋AML）患者及伴有髓系抗原表达型急性淋巴细胞白血病（My＋ALL）患者于治疗一、二疗程后分别行流式细胞术检测,对比检测结果。结果 HAL患者在髓系CD33免疫表型的阳性率最高为95%,在B系CyCD22免疫表型的阳性率最高为75%,在T系CD7免疫表型的阳性率最高为30%,且在CD2、CyCD3、CD5中均有表达。第一、二疗程Ly＋AML与My＋ALL的诱导缓解率均明显高于HAL（P〈0.05或P〈0.01）。结论流式细胞术检测急性杂合性白血病具有较高的阳性检出率,对于临床治疗的效果评估具有重要的指导意义。

Clinical analysis of hybrid acute leukemia detected by flow cytometry

Objective To analyze clinical effect of hybrid acute leukemia detected by flow cytometry. Methods The patients with hybrid acute leukemia （HAL） and acute myelocytic leukemia with lymphocytic antigen expression （Ly＋AML） and acute lymphoblastic leukemia with myeloid antigen expression （My ＋ ALL） in our hospital were detected by flow cytometry after one and two course of treatment respectively, and The test results were compared. Results The maximum positive rate of myeloid CD33 immunophenotype in HAL patients was 95%, the maximum positive rate of B lymphocyte CyCD22 immunophenotype in HAL patients was 75%, the maximum positive rate of T lymphocyte CD7 immunophenotype in HAL patients was 30%, and CD2 , CyCD3 and CD5 were all expressed. The remission induction rate of Ly＋AML and My＋ALL in first and second course of treatment were both higher obviously than that of HAL patients （P 0.05 or 0.01）. Conclusion Hybrid acute leukemia detected by flow cytometry has high positive detective rate. It has important directive to evaluation on the clinical therapeutic effect.