姜黄素与紫杉醇联用对人子宫内膜癌细胞增殖的影响及其机制研究 |
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引用本文: | 杨晓霞,李美蓉. 姜黄素与紫杉醇联用对人子宫内膜癌细胞增殖的影响及其机制研究[J]. 中国当代医药, 2013, 0(17): 21-23 |
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作者姓名: | 杨晓霞 李美蓉 |
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作者单位: | 山西医科大学第一临床医学院妇产科,山西太原030001 |
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摘 要: | 目的探讨姜黄素(CCM)与紫杉醇(PIX)联用对人子宫内膜癌细胞(Ishikawa)增殖的影响及其机制。方法(1)设置对照组(不加药物)、CCM组(不同浓度的CCM)、PIX组、联合组(PIX联合不同浓度的CCM);(2)采用MTT法检测不同浓度的CCM联合PIX对细胞活力的影响;(3)流式细胞术检测细胞凋亡率;(4)免疫组化法检测Caspase-3蛋白表达情况。结果CCM联用PIX能抑制人子宫内膜癌细胞的增殖(P〈0.05),且随CCM浓度的增加,抑瘤作用增加;两种药物均可诱导细胞凋亡,且各组凋亡率与药物浓度呈正相关(P〈0.05);免疫组化结果显示随着染毒浓度的增加和联合作用,Caspase-3蛋白的表达升高(P〈0.05)。两药联用抗瘤作用增强。结论CCM、PIX均可抑制人子宫内膜癌细胞的增殖、诱导细胞凋亡、上调细胞Caspase-3表达,呈剂量依赖性,且两者联用具有协同作用。
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关 键 词: | 姜黄素 紫杉醇 Caspase-3 子宫内膜癌 增殖 凋亡 |
Effect and mechanism on proliferation of curcumin with the combination of paclitaxel on human endometrial carcinoma cell |
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Affiliation: | YANG Xiaoxia ,LI Meirons (Department of Gynaecology and Obstetrics,the First Clinical Medical College of Shanxi Medical University,Taiyuan 030001,China) |
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Abstract: | Objective To investigate the effects and its mechanism of curcumin (CCM) and paclitaxel (PIX) combination of human endometrial carcinoma cell (Ishikawa) proliferation. Methods (1)Set up control group(without any drugs),cur- cumin group(different concentrations curcumin),paelitaxel group, combination group(PIX with different concentrations curcumin);(2)Joint detection of different concentrations of CCM PIX effects on cell viability was determined by MTT method;(3)Cell apoptosis was detected by flow cytometry rate;(4)Immunohistochemistry was used to detect the expres- sion of Caspase-3 protein. Results Curcumin combined with Paelitaxel could inhibit the proliferation of human en- dometrial cancer cells (P 〈 0.05), and with the increase of curcumin concentration,tumor inhibition increased; The two drugs could induce cell apoptosis,and the apoptosis rate and positively related to drug concentration (P 〈 0.05). Im- munohistochemistry results showed that with the increase of exposure and the combined action, increased the expres- sion of Caspase-3 protein(P 〈 0.05). The two drugs combined with antitumor effect enhancement. Conclusion Expres- sion of CCM, PIX can inhibit the proliferation of human endometrial cancer cells, inducing cell apoptosis, upregulation of Caspase-3, in a dose dependent manner, and has a synergistic effect with two. |
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Keywords: | Curcumin Paelitaxel Caspase-3 Endometrial carcinoma Proliferation Apoptosis |
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