姜黄素与紫杉醇联用对人子宫内膜癌细胞增殖的影响及其机制研究

目的探讨姜黄素（CCM）与紫杉醇（PIX）联用对人子宫内膜癌细胞（Ishikawa）增殖的影响及其机制。方法（1）设置对照组（不加药物）、CCM组（不同浓度的CCM）、PIX组、联合组（PIX联合不同浓度的CCM）；（2）采用MTT法检测不同浓度的CCM联合PIX对细胞活力的影响；（3）流式细胞术检测细胞凋亡率；（4）免疫组化法检测Caspase-3蛋白表达情况。结果CCM联用PIX能抑制人子宫内膜癌细胞的增殖（P〈0．05），且随CCM浓度的增加，抑瘤作用增加；两种药物均可诱导细胞凋亡，且各组凋亡率与药物浓度呈正相关（P〈0．05）；免疫组化结果显示随着染毒浓度的增加和联合作用，Caspase-3蛋白的表达升高（P〈0．05）。两药联用抗瘤作用增强。结论CCM、PIX均可抑制人子宫内膜癌细胞的增殖、诱导细胞凋亡、上调细胞Caspase-3表达，呈剂量依赖性，且两者联用具有协同作用。

Effect and mechanism on proliferation of curcumin with the combination of paclitaxel on human endometrial carcinoma cell

Objective To investigate the effects and its mechanism of curcumin （CCM） and paclitaxel （PIX） combination of human endometrial carcinoma cell （Ishikawa） proliferation. Methods （1）Set up control group（without any drugs）,cur- cumin group（different concentrations curcumin）,paelitaxel group, combination group（PIX with different concentrations curcumin）;（2）Joint detection of different concentrations of CCM PIX effects on cell viability was determined by MTT method;（3）Cell apoptosis was detected by flow cytometry rate;（4）Immunohistochemistry was used to detect the expres- sion of Caspase-3 protein. Results Curcumin combined with Paelitaxel could inhibit the proliferation of human en- dometrial cancer cells （P 〈 0.05）, and with the increase of curcumin concentration,tumor inhibition increased; The two drugs could induce cell apoptosis,and the apoptosis rate and positively related to drug concentration （P 〈 0.05）. Im- munohistochemistry results showed that with the increase of exposure and the combined action, increased the expres- sion of Caspase-3 protein（P 〈 0.05）. The two drugs combined with antitumor effect enhancement. Conclusion Expres- sion of CCM, PIX can inhibit the proliferation of human endometrial cancer cells, inducing cell apoptosis, upregulation of Caspase-3, in a dose dependent manner, and has a synergistic effect with two.