EBV—DNA定量检测对鼻咽癌的诊断

目的通过对鼻咽癌患者经治疗后EBV—DNA含量变化的观察，为临床诊治陔病寻求最佳方法。方法血浆EBV—DNA检测采用荧光定量PCR法，选择确诊的145例鼻咽癌患者为研究对象，对其EBV-DNA进行定量检测，并以同期健康者140名的血浆情况进行对比研究。结果鼻咽癌组中有136例（93．79％）患者血浆EBV—DNA呈阳性：健康组中有10例（7．14％）血浆EBV—DNA呈阳性，鼻咽癌组患者出浆EBV—DNA阳性检出率明显高出健康组（P〈0．01）；血浆EBV-DNA呈阳性的患者在治疗过程中，血浆EBY—DNA含量呈逐渐下降趋势。结论给予鼻咽癌患者EBV—DNA定量检测，对诊断和指导治疗价值明显。

Quantitative detection of EBV-DNA for diagnosis of nasopharyngeal carcinoma

Objective To seek the best approach of diagnosis and treatment for the disease through observing the changes of EBV-DNA content of nasopharyngeal carcinoma patients after treatment. Methods The plasma EBV-DNA was detected by fluorescence quantitative PCR method. One hundred and forty five patients confirmed as nasopharyngeal carcinoma were selected as study object. Their plasma EBV-DNA were detected and compared with those of 140 healthy persons in corresponding time. Results In the nasopharyngeal carcinoma group, 136 cases （93.79%） showed plasma EBV-DNA-positive, while 10 cases （7.14%） in healthy group. The plasma EBV-DNA-positive detection rate of nasopharyngeal carcinoma group was significantly higher than that of healthy group （P 〈 0.01）. In plasma EBV-DNA- positive patients, the plasma EBV-DNA content decreased gradually in the course of treatment. Conclusion The quantitative detection of EBV-DNA for nasopharyngeal carcinoma patients has significant value in diagnosing and guiding treatment of the disease.