库存软骨异体移植后的图像定量分析

０．４％戊二醛甲醛混合液保存４周的猪透明肋软骨异体皮下移植１～５月后图像定量分析移植软骨的细胞学形态参数及组织中ＧＡＧ（酸性粘多糖）的含量．结果表明：各实验组细胞密度均高于对照组（ｐ＜０．００１），证明软骨细胞继续保持其增殖分裂活性．保存未移植软骨，移植１月及３月后细胞形态因子、核周长、核面积ＤＮＡ相对含量等４项参数均无显著性差异（ｐ＞０．０５），而移植５月后该４项参数均与对照组有显著差异（ｐ＜０．００１），均不同程度下降达原对照组的８７．８％，８９．４％，７７．０％及７６．８％．核浆比值在保存及异体移植１～３月后升高，移植５月后仍继续升高达对照组的１．３１倍．ＡＢ染色切片细胞内的酸性粘多糖含量于移植１～５月内升高超过对照组含量，于５月末达到对照组的１０８．３％（ｐ＜０．００１），而基质中的ＧＡＧ含量在移植后１～３月时与对照组无明显差异（ｐ＞０．０５）而移植５月后下降（ｐ＜０．００１）但仍达对照组９９．７％．

The Quantative Determination of Image Analysis of Preserved Catilage Allografts

The present paper studied the cell's morphological parameters and the quantities of GAG by image analysis technigue in porcine cartilage histology which were preserved in 0.4% glutaraldehyde and formaldehyde mixture in a duration of 4 weeks,then allografted subdermaly during 1～5 mohths.The result showed:the cell's density of the various experiment groups were all higher than those of the control's(p<0.001).The result suggested that the cartilage cells kept the properity of mitosis and fertility. The 4 parameters about cell's morphological factor,nucler area and DNA reletative quantity among the preserving groups,groups of grafting after 1 month and 3 months were all with no significant difference.(p<0.05).But the 4 parameters of the groups of grafting after 5 months were with significant difference,compared with those of the normal control's(p<0.001),it reduced down to be 87.8%,89.4,77.0% and 76.8% that of the normal controls respectively.