尖锐湿疣的原位杂交组化和免疫组化对比研究

目的　寻找尖锐湿疣的一种较确切的病理诊断方法。方法　对 48例尖锐湿疣 (CA)档案蜡块作连续切片 ,分别作HE、原位杂交组化HPV DNA和免疫组化HPV Ag(HPV衣壳抗原 )染色 ,在光镜下对比观察 ,统计分析。 结果　 3 0例免疫组化阳性病例 ,进行原位杂交组化和重复免疫组化染色均为阳性 ;18例免疫组化阴性病例 ,原位杂交组化 13例 (72 .2 % )阳性 ,重复免疫组化染色 5例 (2 7.2 % )阳性 ;在连续切片上 ,显示原位杂交组化比免疫组化的阳性细胞明显增多。结论　HPV原位杂交组化特异性好 ,灵敏度高 ,方法与免疫组化相似 ,可以在临床病理科推广应用

Study on Condyloma Acuminatum by in situ Hybrihistochemistry Compared with Immunohistochemistry

Objective To seek a appropriate diagnostic method for condyloma acuminatum. Methods 48 cases of condyloma acuminatum were used,serial sectioned and stained with HE,in situ hybrihistochemistry(ISHC) and immunohistochemistry(IHC) respectively,assessed under microscopy and was analysed statistically.Results 30 IHC positive cases were all positive by ISHC and IHC stain,13 cases(72.2%) in 18 IHC negative cases were positive by ISHC stain,5 cases (27.2%) were positive by repeated IHC stain.In the serial sections,ISHC positive cells were more than IHC positive cells. Conclusion HPV ISCH stain is higher special than that of IHC stain,ISCH method is same as IHC,may be widely used in clinical pathology.