高效液相色谱法测定芹菜素在不同血浆中的蛋白结合率

目的:建立测定芹菜素在大鼠血浆、人血浆和牛血清白蛋白(BSA)中蛋白结合率的方法,并计算不同介质中的的相关参数。方法:采用高效液相色谱(HPLC)测定不同介质中芹菜素的浓度,并结合平衡透析法测定蛋白结合率。结果:高、中、低浓度下,芹菜素的血浆蛋白结合率分别为:大鼠血浆:(99.4±3.8)%、(99.6±5.6)%、(99.5±4.5)%;人血浆:(96.3±7.2)%、(97.9±10.5)%、(97.8±9.7)%;牛血清白蛋白:(98.7±8.6)%、(99.6±9.4)%、(99.1±8.0)%。结论:本方法快速、简便、可靠,芹菜素与大鼠血浆、人血浆和牛血清白蛋白结合率很高,且与血药浓度无显著相关性。

Determination of the protein binding rates of apigenin with different kinds of plasma by HPLC

OBJECTIVE To establish a method for determining the protein binding rates of apigenin in rat plasma,human plasma,bovine serum albumin(BSA),and to calculate the correlate related parameters of apigenin in different matrix.METHODS The concentrations of apigenin in different matrix were assayed by high performance liquid chromatography(HPLC).The protein binding rates of apigenin in different matrix were determined by equilibrium dialysis method.RESULTS The protein binding rates of apigenin with rat plasma,human plasma and BSA were(99.4±3.8)%,(99.6±5.6)%,(99.5±4.5)%,respectively,when high concentration of apigenin was used;they were(96.3±7.2)%,(97.9±10.5)%,(97.8±9.7)%,respectively,when medium concentration of apigenin was used;and they were(98.7±8.6)%,(99.6±9.4)%,(99.1±8.0)%,respectively,when low concentration of apigenin was used.CONCLUSION The method was validated to be rapid,and reliable.The protein binding rates of apigenin with rat plasma,human plasma and BSA were very high.Moreover,the plasma protein binding rates were dose-independent.