前列腺癌肿瘤微环境树突状细胞与T细胞免疫的关系

【目的】了解前列腺癌组织树突状细胞与肿瘤微环境T细胞免疫的关系。【方法】对 2 2例前列腺癌组织和周围正常前列腺组织用S10 0、CD3、CD2 1和CD14mAb分别作SP免疫组化染色 ,光镜下对阳性细胞计数并计算细胞指数 ,对癌组织S10 0 和CD3 细胞指数行相关性分析。【结果】前列腺癌组织和正常组织可观察到S10 0 DCs和CD3 T细胞分布 ,而CD2 1 B细胞和CD14 单核 /巨噬细胞均少见。癌组织中S10 0 DCs指数和CD3 T细胞指数均明显少于周围正常组织 (P<0 0 5 ) ,癌组织S10 0 DCs指数与CD3 T细胞指数呈正相关 (r =0 86 ,P <0 0 5 )。【结论】DCs是前列腺癌肿瘤微环境中的主要抗原提呈细胞 ,癌组织DCs数量减少可能与前列腺癌肿瘤微环境T细胞免疫功能低下有关。

The Relationship Between Dendritic Cells and T Lymphocytes Immunity in Tumor Microenvironment of Human Prostate Cancer

Objective To investigate the relationship between dendritic cells and T lymphocytes immunity in tumor microenvironment of human prostate cancer. Methods S100 , CD3 , CD21 , and CD14 cells were determined by immunohistochemical staining (SP method) in 22 cases of prostate cancer and adjacent normal prostate tissue. The amount of positive cells were counted using microscope and the cell index were calculated. Results S100 DCs and CD3 T lymphocytes were detected, while CD14 B lymphocytes and CD21 monocytes/macrophages were rarely detected. The cell index of S100 DCs and CD3 T lymphocyte were less in cancer than in adjacent normal prostate tissue, respectively (P<0.05). There was a significant positive correlation between the cell index of S100 DCs and CD3 T lymphocyte in cancer (r=0.86, P<0.05). Conclusion DCs is the main antigen presenting cells in tumor microenvironment of prostate cancer, and the decrease of DCs number in cancer is related to the decrease of T lymphocyte immunity in tumor microenvironment of prostate cancer.