JNK信号转导通路对白细胞介素-1β促肝星状细胞增殖的调控作用

目的：探讨JNK信号转导通路在白细胞介素- 1β（IL-1β）介导的促肝星状细胞（HSCs）增殖中的作用。方法:应用 Western印迹法检测JNK的活化程度。应用活细胞计数试剂盒-CCK-8检测HSCs增殖并观察JNK 特异性阻断剂SP600125对IL-1β促HSCs增殖的影响。结果：IL-1β有明 显促大鼠HSCs增殖作用，而经JNK特异性阻断剂 SP600125预处理后，IL-1β促HSCs增殖作用 受到抑制（1.560±0.110 vs 1.427±0.113，P<0.05）。IL-1β以时间依赖 方式激活JNK。IL-1β作用HSCs后0、5、15、30、60和120 min，JNK 活性分别为0.982±0 .299、1.501±0.720、2.133±0.882、3.360±0.452、2.181±0.789、1.385±0 .368。结论:IL-1β可刺激HSCs增殖，细胞内JNK信号转导通路参与了 IL-1β促HSCs增殖作用。

Regulatory effects of JNK transduction on interleukin- 1β- induced proliferation in rat hepatic stellate cells

AIM:To study the action of JNK in stimulat ing proliferation in rat hepatic stellate cells (HSCs) induced by interleukin-1β.METHODS:Activation of JNK was detected with Western blotting,w hile the proliferation of HSCs induced by interleukin-1β and the effect of JNK specific blockade SP600125 were measured with cell counting kit-8.RESULTS:Interleukin-1β up-regulated proliferation in rat HSCs,which was obviously inhibited by SP600125,compared with control group (without SP600125 treatment) (1.560±0.110),the decrease was significant (1.427±0.113,P<0.05).Interleukin-1β activated JNK signaling pathway in a time-de pendent manner.At the six time points (0,5,15,30,60 and 120 min),the JNK a ctivities were 0.982±0.299,1.501±0.720,2.133±0.882,3.360±0.452,2.181±0.789,1.385±0.368,respectively.CONCLUSION:Interleukin-1β stimulates proliferation in rat HSCs ,and JNK signaling pathway is involved in this process.