| 食品中幽门螺杆菌荧光PCR快速检测方法的建立及评价 |
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| 引用本文： | 刘静秋,史艳宇,刘金华,邴炜,华蕾,杨波,朱颖,王洪昌.食品中幽门螺杆菌荧光PCR快速检测方法的建立及评价[J].吉林大学学报(医学版),2011,37(1):175-178. |
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| 作者姓名： | 刘静秋 史艳宇 刘金华 邴炜 华蕾 杨波 朱颖 王洪昌 |
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| 作者单位： | 吉林省产品质量监督检验院,吉林,长春,130022;吉林出入境检验检疫局,吉林,长春,130062 |
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| 基金项目： | 国家质量监督检验检疫总局资助课题 |
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| 摘 要： | 目的:建立一种快速、特异、灵敏的幽门螺杆菌检测方法,并对方法进行评价,为快速、准确、有效检测幽门螺杆菌方法的建立提供依据.方法:以尿素酶基因序列为靶位点,用Primer Express 3.0软件设计引物及探针,经Blast软件对相似序列搜索后,筛选出一套最优与常见致病菌无交叉反应的引物;分别用食品中常见致病菌大肠杆菌...
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| 关 键 词： | 幽门螺杆菌 尿素酶基因 荧光PCR 食品 |
| 收稿时间： | 2010-06-23 |
Establishment and evaluation of real time PCR for detection of Helicobacter pylori in food |
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| LIU Jing-qiu,SHI Yan-yu,LIU Jin-hua,BING Wei,HUA Lei,YANG Bo,ZHU Ying,WANG Hong-chang.Establishment and evaluation of real time PCR for detection of Helicobacter pylori in food[J].Journal of Jilin University: Med Ed,2011,37(1):175-178. |
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| Authors: | LIU Jing-qiu SHI Yan-yu LIU Jin-hua BING Wei HUA Lei YANG Bo ZHU Ying WANG Hong-chang |
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| Institution: | 1.Jilin Product Quality Supervision Inspection,Changchun 130022,China；2.Jilin Entry-Exit Inspection and Quarant Bureau,Changchun 130062,China |
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| Abstract: | Objective To establish a rapid,specific and sensitive method for the detection of Helicobacter pylori,and provide an effective detecting evidence for Helicobacter pylori.Methods A pair of primers and probe corresponding to the urease gene for real time PCR were designed according to Primer Express 3.0 software,similar sequences were searched by Blast method,and the excellent primers and probe were selected.DNA from common bacterial pathogens such as Escherichia coli,Staphylococcus,Listeria monocytogenes,Cam... |
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| Keywords: | Helicobacter pylori urease gene real time PCR food |
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