| 人血浆中水飞蓟宾浓度的HPLC测定法 |
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| 引用本文： | 吴刚,高丹.人血浆中水飞蓟宾浓度的HPLC测定法[J].黑龙江医学,2005,29(8):578-579. |
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| 作者姓名： | 吴刚 高丹 |
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| 作者单位： | 1. 佳木斯大学药学系,黑龙江,佳木斯,154007
2. 哈药集团三精制药有限公司,黑龙江,哈尔滨,150001 |
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| 摘 要： | 目的建立人血浆中水飞蓟宾浓度的测定法。方法采用高效液相色谱法,μBondapak(300mm×4.6m,5μm)色谱柱,及μBondapak预柱(10mm×4.6mm,5μm)为分析柱。乙晴、水和二氧六环(20:70:10)混合溶剂为流动相,流速为1.0mL/min,检测波长为288nm,柱温为40℃。结果水飞蓟宾在5.123～327.9μg/mL范围内线性良好(r=0.9998),方法回收率为93.2%～96.8%,日内及日间RSD<10%。结论方法简便快速、灵敏度高,适合于临床药学研究需要。
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| 关 键 词： | 药理学 水飞蓟宾 血浆浓度 高效液相色谱法 |
| 文章编号： | 1004-5775(2005)08-0578-02 |
| 收稿时间： | 05 10 2005 12:00AM |
| 修稿时间： | 2005年5月10日 |
Determination of Silybin in Human Plasma by HPLC |
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| WU Gang,GAO Dan.Determination of Silybin in Human Plasma by HPLC[J].Heilongjiang Medical Journal,2005,29(8):578-579. |
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| Authors: | WU Gang GAO Dan |
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| Abstract: | Objective To establish a precise, simple and convenient method for the determination of sylybin concentration in human plasma. Methods HPLLC system was used u Bondapak (300 mm×4.6 um) and (10 mm×4.6 um) as the column, mixture of acetonitrile, water and dioxane as the mobile phase. The flow rate of 1.0 mL/min and column temperature of 40℃ was adopted and the detection wavelength of 288 nm was specified. Results The calibration curve was linear over the range from 5.123 to 327.9 ng/mL, with r=0.9998. The recovery was 93.2～96.8%. The RSD for within day variation and the between-day variation were less than 10%. Conclusion The method might be simple, precise and sensitive and apply conveniently in studies of clinical pharmacy. |
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| Keywords: | Silybin Plasma concentration HPLC |
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