| 氯通道阻滞剂在β_(25-35)淀粉样多肽诱导PC12细胞凋亡的保护作用 |
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| 引用本文： | 黄春霞,李源,龚卫琴,张英起,张志敏,啜玉彩. 氯通道阻滞剂在β_(25-35)淀粉样多肽诱导PC12细胞凋亡的保护作用[J]. 中华老年心脑血管病杂志, 2008, 10(4): 304-306 |
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| 作者姓名： | 黄春霞 李源 龚卫琴 张英起 张志敏 啜玉彩 |
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| 作者单位： | 第四军医大学西京医院，第四军医大学西京医院，第四军医大学西京医院，第四军医大学生物制药教研室，第四军医大学西京医院，第四军医大学西京医院 |
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| 基金项目： | 陕西省自然科学基金(2006C212) |
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| 摘 要： | 目的探讨氯通道阻滞剂(DIDS)对β25-35淀粉样多肽(Aβ25-35)诱导PC12细胞凋亡的影响。方法PC12细胞按实验不同分为对照组(DMEM培养液)、Aβ25-35组(Aβ25-35 40μmol/L)、DIDS组(Aβ25-35 40μmol/L+DIDS 50μmol/L)。MTT法测细胞存活率,Hoechst 33258荧光染色观察细胞核形态学改变,流式细胞技术测细胞凋亡率。结果Aβ25-35组与对照组和DIDS组比较,PC12细胞的存活率明显降低(P<0.05),细胞凋亡率明显增高(P<0.05),不同DIDS浓度依赖性升高PC12细胞存活率,DIDS 50μmol/L能显著下调Aβ25-35诱导的细胞凋亡(P<0.05)。结论DIDS对Aβ25-35诱导PC12细胞损伤有保护作用。
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| 关 键 词： | 阿尔茨海默病 氯化物通道 淀粉样β蛋白 细胞凋亡 PC12细胞 |
| 文章编号： | 1009-0126(2008)04-0304-03 |
| 修稿时间： | 2007-08-27 |
Protective effect of Cl-channel blockers DIDS against Aβ_(25-35)-induced PC12 cell apoptosis |
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| HUANG Chun-xia,LI Yuan,GONG Wei-qin,et al. Protective effect of Cl-channel blockers DIDS against Aβ_(25-35)-induced PC12 cell apoptosis[J]. Chinese Journal of Geriatric Cardiovascular and Cerebrovascular Diseases, 2008, 10(4): 304-306 |
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| Authors: | HUANG Chun-xia LI Yuan GONG Wei-qin et al |
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| Abstract: | Objective To study the effects of Cl-channel blockers DIDS on Aβ25-35-induced apoptosis.Methods The PC12 cells were divided into 3 groups:the control group,the Aβ25-35 group,the DIDS protective group.MTT assay was used to detect the cellular viability.Hoechst 33 258 fluorescence staining and flow cytometric analysis were used to detect the apoptosis.Results Cellular viability decreased and the apoptotic rate increased after PC12 cell was treated with Aβ25-35.DIDS attenuated Aβ25-35-induced PC12 cell apoptosis in a dose-dependent manner and 50 μmol/L had the significant protective effect(P<0.05).Conclusion DIDS protects PC12 cells from apoptosis induced by Aβ25-35. |
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| Keywords: | Alzheimer disease chloride channels amyloid beta-protein apoptosis PC12 cell |
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