蟾酥注射液对小鼠S180和人结肠癌HT-29裸鼠移植性肿瘤的影响

目的研究蟾酥注射液对小鼠移植性肿瘤 S180和人结肠癌 HT-29裸鼠移植性肿瘤的抑制作用.方法分别用小鼠 S180和人结肠癌 HT-29裸鼠两种荷瘤小鼠模型,观察药物对上述肿瘤的抑瘤作用,并镜下观察后者细胞凋亡情况.结果与荷瘤阴性对照组比较,蟾酥注射液各剂量组对小鼠 S180抑瘤率( IR)为 19.1%～38.2%(P<0.05),呈量效关系;而对人结肠癌 HT-29裸鼠移植性肿瘤的 IR为 9.5%～15.8%(P>0.05),也呈量效关系,但差异均未见统计学意义;环磷酰胺则能显著抑制小鼠 S180和 HT-29细胞裸鼠移植性肿瘤的生长( IR分别为70.7%和 67.1%, P<0.01),镜检可见其有显著促进肿瘤细胞凋亡作用;未发现实验药物出现明显的毒副作用.结论该实验所用的蟾酥注射液,对小鼠 S180有抑制作用,而对人结肠癌 HT-29裸鼠移植性肿瘤,则作用不明显,表明不同类型的肿瘤对其敏感性不同.

Effects of Chansu injection on transplanting-tumor models S180 in mice and human colon cancer HT-29 in nude mice

OBJECTIVE: To study the antitumor effects of Chansu injection on transplanting- tumor of S(180 ) in mice and human colon cancer HT-29 in nude mice. METHODS: Using transplanting- tumor models of S(180 ) in mice and human colon cancer HT-29 in nude mice,the tumor inhibitive ratio(IR) of Chansu injection was determined and apoptosis was microscopically observed. RESULTS: Compared with tumor-negative control groups, IR at different dosage of Chansu in models of S(180) and HT-29 was 19.1% - 38.2% and 9.5% - 15.8% respectively,there was a dose-dependent relationship in models of S ( 180) (P< 0.05) and HT- 29 (P> 0.05). The tumor growth was markedly inhibited by cyclophosphamide (CTX) in model of S( 180) with IR of 70.7% and in model of HT-29 with IR of 67.1%, compared with control groups, both P< 0.01; apoptosis induced by CTX was markedly observed by in microscope examination. No significant side effects were shown in the study group. CONCLUSIONS: Chansu injection can significantly inhibit tumor growth in model of S(180), but not in model of HT- 29. Different type of tumor has different drug-sensitivity.