食管鳞状细胞癌中nm23-H1和p53蛋白表达及其相互关系

目的:探讨食管鳞癌组织中p53和nm23-H1蛋白的表达与癌组织分化浸润转移的关系,以及探讨两者之间的相关性,并进一步分析癌组织中p53和nm23-H1蛋白表达对食管癌患者的预后意义。方法:采用免疫组织化学(S-P法)方法对100例人食管鳞癌组织中的p53和nm23-H1蛋白的表达情况进行检测。结果:100例食管鳞癌组织中,nm23-H1阳性表达者70例(阳性率为70%),p53阳性表达者64例(阳性率为64%)。nm23-H1蛋白表达与食管癌淋巴结转移有关(P<0.025),与食管鳞状细胞癌的分化程度、肿瘤部位、浸润深度、病变长度以及患者性别、年龄无关(P>0.05)。p53蛋白表达与食管鳞状细胞癌的分化程度、浸润深度有关(P<0.05),与食管癌淋巴结转移、肿瘤部位、病变长度、患者性别、年龄无关(P>0.05)。高分化鳞癌组织中p53明显低表达(29.2%);低分化鳞状细胞癌组织中p53表达明显增高(71.4%)。食管外膜受累者p53表达较高(56%);仅发生食管粘膜和(或)粘膜下浸润组的癌组织中未发现有p53蛋白的表达。食管癌组织中nm23-H1蛋白低(高)表达与p53高(低)表达之间有明显相关性(P<0.01)。nm23-H1和p53蛋白表达亦与食管癌的TNM分期密切相关(P<0.05)。食管癌TNM分期越晚,其癌组织中nm23-H1蛋白表达越低,p53蛋白表达越高。结论:nm23-H1基因低表达与p53基因高表达可能在食管鳞状细胞癌浸润转移过程中发挥重要作用。nm23-H1可以作为食管鳞状细胞癌患者预后的基因标记,其蛋白表达产物的检测可以用于患者预后的判断,并为患者治疗方案的制定提供参考。

The correlation of p53 and nm23-H1 expression in squamous carcinoma of esophagus

Objective:To study the relationship between expression of p53and nm23-H1 and differentiation,invasiveness and metastasis in human esophageal squamous carcinoma,and the correlation between expression of p53and nm23-H1.Methods:Expression of p53and nm23-H1 in 100 patients with squamous cell carcinoma of esophagus were studied by using immunohistochemical S-P methods.Results:Serenty cases(70%) and 64 cases(64%) of esophageal squamous cell carcinoma were positive in the examination of nm23-H1 protein and p53protein,respectively.The expression of nm23-H1 was related to lymph nodes metastasis(P<0.025),but not related to tumor differentiation,invasiveness,tumor location,tumor length,patient's gender and age(P>0.05).The lymph nodes metastasis positive group had very lower expression of nm23-H1,with negative rate 70.8%.But the negative group had a higher nm23-H1 expression(65.4%).The expression of p53 was related to tumor differentiation and invasiveness(P<0.05),but not related to lymph nodes metastasis,tumor location,tumor length,patient's gender and age(P>0.05).Among the three groups,the highest differentiation group had the lowest expression of p53,with the positive rate 29.2%,but the low differentiation group had the highest p53 positive rate(71.4%).As for tumor invasiveness,the group of outer membrane of esophagus infiltrated had the highest p53 protein positive rate(56%),and the group of mucous or submucous layer infiltrated was not detected p53protein.The low expression of nm23-H1 and the high expression of p53were also correlated.The expression of nm23-H1 and p53were both correlated with TNM stage of esophageal carcinoma(P<0.05).The better esophageal carcinomas differentiated,the more lowly nm23-H1 expressed and the more highly p53 expressed.Conclusion:Low expression of nm23-H1 and high expression of p53 play an important role in the progression of squamous cell carcinoma of esophagus.Nm23-H1 might be a gene marker in the prediction of patients' prognosis,and helpful in treatment.