缝隙连接蛋白43和胰岛素样生长因子Ⅰ与脑胶质瘤恶性程度的相关性研究

目的 探讨缝隙连接蛋白43（cx43）、胰岛素样生长因子Ⅰ（IGF-Ⅰ）和细胞核相关抗原Ki-67在人脑胶质瘤中的表达及相关性。方法 采用免疫组织化学SP法检测45例胶质瘤组织和10例正常脑组织中Cx43、IGF-Ⅰ、Ki-67的表达。结果 Cx43在Ⅰ-Ⅱ级脑胶质瘤中阳性表达率为87．5％，Ⅲ-Ⅳ级中的阳性表达率为42．9％，两者间的差异有显著性（P〈0．01），且随Ki-67表达升高而降低（r=-0．893，P〈0．01）；IGFⅠ在Ⅰ-Ⅱ级脑胶质瘤中的表达（阳性率58．3％）与在Ⅲ-Ⅳ级（阳性率85．7％）中的表达差异有显著性（P〈0．05），且与Cx43的表达呈明显负相关（r=-0．688，P〈0．01）。结论 Cx43表达水平的下调或缺失不是胶质瘤发生的始动因素，可能是胶质瘤恶性演进过程中的一个重要分子事件，促使肿瘤细胞恶性增殖程度增加。

Relationship of Cx43 and IGF-Ⅰ with Malignancy Degree of Human Glioma

Objective To explore connexin 43（Cx43） gene, insulin-like growth fator-Ⅰ （IGF-Ⅰ ） and Ki-67 expressions in human glioma and the relationship among them. Methods Expressions of Cx43, IGF-Ⅰ and Ki-67 were determined by immunohistochemistry staining in 45 cases of gliomas and 10 cases of normal brain tissues. Results The positive rate of Cx43 expression was 87.5% （21124） in th..e low grade （WHO grade Ⅰ -Ⅱ ） gliomas, and 42.9%（9121） in the high grade （WHO grade Ⅲ-Ⅳ） gliomas. There was significant difference in the positive rate of Cx43 expression between the low and high grades gliomas （P〈0.01）.In the gliomas the expression of Cx43 was negatively related with Ki-67 expression （r=-0.893, P〈0.01）. The positive rate of IGF-Ⅰ expression in the low grade gliomas （58.3%, 14124） was significantly lower （P〈0.05） than that in the high grade gliomas （85.7%, 18/21）. In the gliomas the IGF-Ⅰ expression was negative related with Cx43 expression （r=-0.688, P〈0.01）. Conclusion The deficiency or down regulation of Cx43 expression is not a factor producing gliomas occurrence,but maybe it promotes proliferation and development of the glioma cells.