琼胶寡糖抑制血管形成作用的研究

目的:探讨琼胶寡糖(AOS)对血管形成的抑制及其作用机制。方法:采用鸡胚尿囊膜模型(CAM)并结合组织化学方法观察AOS抑制血管发育的情况;以人脐静脉内皮细胞(HUVEC)为研究对象,采用MTT法测定AOS的细胞毒情况,并利用Hoechst染色及流式细胞术检测寡糖的促凋亡现象。结果:AOS可明显抑制鸡胚尿囊膜的微血管形成,呈量效关系,在200μg/egg时,抑制率达50.98%;AOS对各类细胞的细胞毒作用有差异,以HUVEC的敏感性较强,在125μg/ml时增殖抑制率达39.66%。AOS有明显的促进HUVEC细胞凋亡的作用,流式细胞仪检测发现AOS能将细胞周期阻滞在S期。结论:琼胶寡糖具有明显的抑制血管形成作用,且该作用主要是通过促进脐静脉内皮细胞凋亡并阻滞细胞周期于S期产生的。

STUDY ON THE ANTI-ANGIOGENIC ROLE OF AGARO-OLIGOSACCHARIDE

Objective: To study the inhibition effect of agaro-oligosaccharide (AOS) on neovascularization and its mechamism. Method: The anti-angiogenic effect in vivo was evaluated on chicken chorioallantoic membrane (CAM) model. Cytotoxic activity of AOS was demonstrated by inhibition of several human cell lines by MTT assay. The apoptosis of HUVECs induced by AOS was examined by Hoechst staining assay and quantified by flowcytometry. Results: In CAM, AOS caused dose-dependent decrease on the vascular density and adversely affected capillary plexus formation. Different cytotoxic sensitivities were observed for AOS towards several kinds of cell lines, and HUVECs were more sensitive. Moreover, the growth inhibitory activity was correlated with induction of apoptosis. Flowcytometric analysis also revealed that AOS arrested the cell cycle progression at S phase. Conclusion: Agaro-oligosaccharide possesses the anti-angiogenic effects, which are associated with apoptosis induction of endothelial cells.