Impact of hepatitis C virus (HCV) genotypes on quantification of HCV RNA in serum by COBAS AmpliPrep/COBAS TaqMan HCV test, Abbott HCV realtime assay [corrected] and VERSANT HCV RNA assay |
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Authors: | Tuaillon Edouard Mondain Anne-Marie Ottomani Laure Roudière Laurent Perney Pascal Picot Marie-Christine Séguret Fabienne Blanc François Larrey Dominique Van de Perre Philippe Ducos Jacques |
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Institution: | Unité Hépatites Virales, Laboratoire de Virologie, Hôpital Lapeyronie,1. Service de Médecine Interne E,2. Institut des Maladies de l''Appareil Digestif, Hôpital Saint-Eloi,3. Département de l''Informatique Médicale Hôpital Arnaud de Villeneuve, CHU de Montpellier, France4. |
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Abstract: | The VERSANT HCV RNA 3.0 (bDNA), COBAS AmpliPrep/COBAS TaqMan HCV, and Abbott ART HCV RealTime assays were compared for hepatitis C virus RNA quantification in 158 clinical specimens (genotypes 1 to 5). RNA values differed significantly between methods (P < 0.0001), and mean titer differences ranged from 0.01 to 0.50 log(10) IU/ml depending on the genotypes. |
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