| 抗人T细胞单克隆抗体与商陆毒蛋白结合物的制备及其抗白血病细胞活性 |
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| 引用本文： | 褚嘉佑,杨爱德,王辨明,胡忠,张和君,史良如,朱晓梅,曲京华,罗兰英,郭仁. 抗人T细胞单克隆抗体与商陆毒蛋白结合物的制备及其抗白血病细胞活性[J]. 华中科技大学学报(医学版), 1989, 0(3) |
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| 作者姓名： | 褚嘉佑 杨爱德 王辨明 胡忠 张和君 史良如 朱晓梅 曲京华 罗兰英 郭仁 |
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| 作者单位： | 同济医科大学血液病研究所(褚嘉佑，杨爱德，王辨明)，中国科学院昆明植物研究所(胡忠，朱晓梅，罗兰英)，中国医学科学院医学生物学研究所(张和君，曲京华)，卫生部武汉生物制品研究所(史良如)，中国医学科学院医学生物学研究所(郭仁) |
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| 摘 要： | 将美洲商陆抗病毒蛋白通过二硫键与抗人T细胞单克隆抗体Wu71偶联制备免疫毒素。结合物经SDS聚丙烯酰胺凝胶电泳、免疫双扩散和间接荧光检测,表明该结合物对人T淋巴细胞白血病CEM细胞显示很强的杀伤作用,在10~(-9)mol/L浓度下可杀灭76.4%的靶细胞,而对抗原阴性的SP_2/O细胞杀灭作用很弱。~(14)C-亮氨酸掺入蛋白质合成抑制试验显示结合物在10~(-9)mol/L时可抑制72.4%的蛋白质合成。这一免疫毒素有可能用于白血病自体骨髓移植时白血病细胞的体外清除。
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| 关 键 词： | 商陆抗病毒蛋白 免疫毒素 白血病细胞 |
Preparation of Monoclonal Anti-human T Cell Antibody and PAP-s Conjugate and Their Selective Anti-Leulcemic Cells Cytotoxic Properties |
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| Chu Jiayou,Yang Aide,Wang Bianming,Hu Zhong Zhang Hejun,Shi Liangru,Zhu Xiaomei,Qu Jinghua,Luo Lanying,Guo Ren. Preparation of Monoclonal Anti-human T Cell Antibody and PAP-s Conjugate and Their Selective Anti-Leulcemic Cells Cytotoxic Properties[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1989, 0(3) |
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| Authors: | Chu Jiayou Yang Aide Wang Bianming Hu Zhong Zhang Hejun Shi Liangru Zhu Xiaomei Qu Jinghua Luo Lanying Guo Ren |
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| Affiliation: | Chu Jiayou,Yang Aide,Wang Bianming,Hu Zhong Zhang Hejun,Shi Liangru,Zhu Xiaomei,Qu Jinghua,Luo Lanying,Guo Ren. Institute of Hematology,Tongji Medical University,Wuhan Kunming Institute of Botany,the Academy of Sciences of China Institute of Medical Biology,Chinese Academy of Medical Sciences Wuhan Institute of Biological Products |
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| Abstract: | Pokeweed antiviral protein(PAP-s) was prepared from seeds of Phyto-lacca americana. Monoclonal antibody against human pan-T lymphocyte Wu7l was linked to PAP-s by a disulfidc bond. The results of SDS-PAGE, double immunodif-fusion in agar,and indirect immunofluorescence test demonstrated that the conjugate consisted of active monoclonal antibody and PAP-s. The conjugate was highly cytotoxic to the human T-leukemic cell line CEM, but not to antigen negative cell line SP2/O. At a concentration of 10-9 mol/L, 76.4% target cells were killed, as compared with 10.1% at 10-8 mol/L of free PAP-s. Treatment of the CEM cells with the conjugate at 10-9 mol/L reduced their rate of protein synthesis by 72.4%, determined with 14C-Leucine incorporation. The immunotoxin may be useful for the in vitro eradication of leukemic cells in autologous marrow transplantation to leukemia patients. |
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| Keywords: | PAP-s immunotoxin leukemic cells |
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