23-羟基白桦酸诱导LoVo细胞凋亡与线粒体膜电位的变化

目的探讨23-羟基白桦酸对人结肠癌细胞株LoVo细胞增殖和凋亡的影响。方法采用MTT法检测23-羟基白桦酸对LoVo细胞的增殖抑制作用。Hoechst染色、流式细胞仪检测细胞凋亡及线粒体膜电位的变化。结果23-羟基白桦酸能抑制LoVo细胞的增殖,其作用呈明显的时间和剂量依赖性。Hoechst33258染色显示细胞凋亡特征。23-羟基白桦酸25、50、100及200μmol·L-1作用LoVo细胞48h后,其凋亡率分别为11.32%±0.92%、19.23%±0.78%、24.51%±5.88%及42.22%±2.32%,显示一定的剂量依赖性关系。与空白组相比,23-羟基白桦酸可明显降低LoVo细胞线粒体膜电位(P<0.01)。结论23-羟基白桦酸可抑制LoVo细胞的增殖,其机制与降低线粒体膜电位继而诱导LoVo细胞凋亡有关。

Apoptotic activity of 23-Hydroxybetulinic acid and the change of mitochondrial membrane potential in human colorectal cancer cell line LoVo

Aim To investigate the apoptotic effects of 23-hydroxybetulinic acid in human colorectal cell line LoVo and the mechanism of apoptosis. Methods MTT-based cytotoxicity assay was used to test the LoVo cell proliferation. The apoptotic cells and the mitochondrial membrane potential were detected by fluorescent miroscopy, flow cytometric analysis. Results 23-Hydroxybetulinic acid inhibited LoVo cell proliferation in a dose and time-dependent manner. Hochst33258 images showed that most cells exposed to 23-hydroxybetulinic acid exhibit apoptotic morphology change. By FCM methods, the apoptotic rates of LoVo cells were 11.32%±0.92%, 19.23%±0.78%, 24.51%±5.88%, 42.22%±2.32%, after treatment of 23-hydroxybetulinic acid at concentrations 25, 50, 100, 200 μmol·L-1, which showed obvious concentration-dependent relationship. Compared with control group, 23-hydroxybetulinic acid caused a concomitant dissipation of the mitochondrial membrane potential, which resulted in LoVo cell apoptosis. Conclusion 23-Hydroxybetulinic acid could inhibit LoVo cell proliferation. The dissipation of mitochondrial membrane potential may result in the LoVo cell apoptosis.