| 甲基莲心碱对耐阿霉素人乳腺癌细胞多药耐药性的逆转 |
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| 引用本文： | 曹建国,唐小卿,周虹,彭波. 甲基莲心碱对耐阿霉素人乳腺癌细胞多药耐药性的逆转[J]. 中德临床肿瘤学杂志, 2004, 3(2): 93-96. DOI: 10.1007/s10330-003-0215-7 |
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| 作者姓名： | 曹建国 唐小卿 周虹 彭波 |
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| 作者单位： | 曹建国 (衡阳南华大学医学院肿瘤研究所,421001); 唐小卿 (衡阳南华大学医学院肿瘤研究所,421001); 周虹 (衡阳南华大学医学院肿瘤研究所,421001); 彭波 (衡阳南华大学医学院肿瘤研究所,421001); |
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| 摘 要： |
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| 关 键 词： | 甲基莲心碱 耐阿霉素 人类 乳腺癌细胞 多药耐药性 逆转作用 细胞毒性 |
| 收稿时间： | 2003-11-20 |
Reversal of Multidrug Resistance by Neferine in Adriamycin Resistant Human Breast Cancer Cell Line MCF-7/ADM |
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| Cao?Jianguo?Email author,Zhou?Hong,Peng?Bo,Tang?Xiaoqing. Reversal of Multidrug Resistance by Neferine in Adriamycin Resistant Human Breast Cancer Cell Line MCF-7/ADM[J]. The Chinese-German Journal of Clinical Oncology, 2004, 3(2): 93-96. DOI: 10.1007/s10330-003-0215-7 |
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| Authors: | Cao?Jianguo? author-information" > author-information__contact u-icon-before" > mailto:caojianguocaojiangguo@hotmail.com" title=" caojianguocaojiangguo@hotmail.com" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,Zhou?Hong,Peng?Bo,Tang?Xiaoqing |
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| Affiliation: | (1) Institute of Oncology, Nanhua University, 421001 Hengyang, China;(2) Department of Physiology, Zhongshan Medical College, Sun Yat-sen University, 512008 Guangzhou, China |
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| Abstract: | Objective: To study the reversal effect of neferine on adriamycin (ADM) resistant human breast cancer cell line MCF-7/ADM. Methods: The cytotoxic effect of Nef or ADM was determined by 3-[4, 5-dimethylthiazol-2.-yl], 5-diphenyl tetraxolium bromid (MTT) assay. Apoptosis and the expression of P-glycoprotein (P-gp) were detected by flow cytometry (FCM). The intracellular ADM concentration was measured by HPLC. Results: Nef at 1, 5, 10  mol/L decreased the IC50 of ADM to MCF-7/ADM from 11.63 g/mL to 4.59, 2.44, 0.27 g/mL respectively. MCF-7/ADM could resist the apoptosis induced by ADM while Nef (1-10 mol/L) could augment ADR-mediated apoptosis. Nef (10 mol/L) increased the accumulation of ADM up to 2.88 fold in MCF-7/ADM but not in sensitive cells MCF-7/S and reduced the expression of P-gp in MCF-7/ADM cells. Conclusion: Nef can circumvent multidrug resistance (MDR) of MCF-7/ADM cells and the mechanism was associated with the increase of intracellular accumulation of ADM and the reduced expression of P-gp in MCF-7/ADM cells. |
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| Keywords: | neferine multidrug resistance adriamycin MCF-7/ADM cells |
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