类肌钙蛋白和钙调蛋白结合蛋白在胃肠运动及其病理适应性调节中的作用

目的；研究类肌钙蛋白（calponion,CaP)和钙调蛋白结合蛋白（caldesmon,CaD)在胃肠道中的表达及其在胃肠运动病理适应性调节中的作用。方法：通过建立慢性番泻叶诱导的胃肠运动增强和实验性肝硬化胃肠运动减弱2种动物模型，应用SDS－PAGE和Western blot方法检测不同胃肠运动状态下胃肠道平滑肌组织中CaP和CaD的表达，并用图像分析进行定量比较。结果：建立了慢性番泻叶诱导的胃肠运动增强和实验性肝硬化胃肠运动减弱2种动物模型。正常小鼠和大鼠胃肠道组织中CaP和CaD的含量依次为结肠＞胃＞小肠；慢性胃肠运动增强小鼠肠道组织中CaP和CaD的含量下降；实验性肝硬化胃肠运动减弱大鼠胃肠道组织中CaP和CaD的含量显著增加，而经左旋硝基精氨酸甲基酯治疗胃肠运动恢复后，它们的含量亦恢复至正常。结论：CaP和CaD的表达与胃肠道各部分的运动状态密切相关，胃肠道运动增强，其含量减少，胃肠道运动减弱则其表达增高。提示这2种蛋白质分子参与了胃肠运动的调节和病理适应性调节过程。

The effect of calponin and caldesmon in regulation of the gastrointestinal motility during pathophysiological adaptation

Objective To investigate the expression of calponin(CaP) and caldesmon(CaD) in the gastrointestinal tract and their effect in regulating gastrointestinal motility during physiological and pathological adaptation .Methods Models of chronic gastrointestinal motility hyperfunction in mice were induced by intragastric administration of senna extraction and models of chronic gastrointestinal motility hypofunction in rats were established with carbon tetrachloride induced cirrhosis , CaP and CaD were detected in the gastrointestinal tract of different model groups using SDS PAGE and Western blot. The color development of Western blots was scanned using densitometric scanning. The relative contents of gastrointestinal CaP and CaD were compared with control animals with different state of gastrointestinal motility.Results Animal models of abnormal gastrointestinal motility were established in mice and rats. Densitometric quantification of CaP and CaD blots by CP1 and C98 mAbs showed that normal animal colon contained higher amounts of h1 CaP and CaD. In normal mice and rats, the content of CaP and CaD was successively in this order :colon>stomach>small intestine .The content was reduced in mice of chronic gastrointestinal motility hyperfunction ,but the expression of CaP and CaD was promoted in rats with cirrhosis and declined to normal level after treatment with L NAME.Conclusion There is close relation between expression of CaP and CaD and state of gastrointestinal motility. CaP and CaD may inhibit gastrointestinal motility. These suggest that CaP and CaD may play a role in the regulation of gastrointestinal motility during physiological and pathological adaptation