人类白细胞抗原-G与胎膜早破关系的研究

目的探讨人类白细胞抗原-G(HLA-G)与胎膜早破(PROM)关系及临床意义。方法随机选取2011年7月至2013年7月临产前行剖宫产分娩的初产妇,检测肘静脉血、羊水及胎膜,分为正常完好胎膜组、足月胎膜早破组和足月前胎膜早破组。应用双抗体夹心酶联免疫吸附实验测定组对象血清、羊水中HLA-G的浓度,比较各组间的相关性。结果正常完好胎膜产妇体内的HLA-G水平以及表达率均比胎膜早破产妇高,并且无感染的正常妊娠产妇胎膜的HLA-G表达率明显比亚临床感染要高,两者经检验均具显著性差异(P0.05)。结论 HLA-G在胎盘中的低表达可能与孕妇感染有关,而HLA-G在胎盘中的低表达可能又与胎膜早破的发生有关,这对于HLA-G在PROM上的研究有着非常重要的意义。

Research on the relationship of HLA -G and premature rupture of membranes

Objective To study the relationship of HLA-G and premature rupture of membranes,and clinical significance. Methods Randomly selecting patients in our hospital in 2011 July to 2013 July. All these patients would receive cesarean delivery. The elbow venous blood, amniotic fluid and amniotic membranes grouping before delivery were collected. Antibody sandwich enzyme-linked immunosorbent was used to determine the amniotic fluid serum concentrations of HLA-G. The correlation between each group was compared. Results HLA-G level of nor-mal intact amniotic membranes of maternal body and expression rate are statistically better than the premature rupture of membranes. And HLA-G expression in normal pregnancy,maternal fetal membranes and non-infected was significantly higher than subclinical infection. All these com-parisons showed significant difference（ P 〈0. 05）. Conclusion Low expression of HLA-G in the placenta may be associated with infection in pregnant women. Low expression of HLA-G in the placenta may be related to the occurrence of premature rupture of membranes,which has a ver-y important for the study of HLA-G on the PROM significance.