| 摘 要： | AIM:To investigate the resistance rate of Helicobacterpylori(Hpylori)to clarithromycin,metronidazole,amoxicillin andtetracycline to guide clinical practice,and to study themechanism of Hpyloriresistant to clarithromycin.METHODS:Thirty Hpylori strains were isolated from themucosa of peptic ulcer,gastric tumor and chronic gastritispatients,then the minimal inhibitory concentration(MIC)toclarithromycin,metronidazole,amoxicillin and tetracyclinewas evaluated by E-test method.The sequence analysis ofPCR fragments was conducted in 23S rRNA gene of Hpyloriresistant to darithromycin to get the resistance mechanismof the bacteria.RESULTS:Among 30 Hpyloristrains,7 cases were resistantto clarithromycin,12 to metronidazole,2 to tetracycline andno strain was found to be resistant to amoxicillin.Theresistance rates were 23.3%,40%,6.7% and 0%,respectively.Three new mutation points were found to berelated to the clarithromycin resistance in Hpyloriisolates,which were G2224A,C2245T and T2289C.CONCLUSION:In northeast China,Hpylorishows highresistance to metronidazole,while sensitive to amoxicillin.The mechanism of resistance to clarithromycin may be relatedto the mutation of G2224A,C2245T and T2289C in the 23SrRNA gene.
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