Rapid molecular genetic assay for direct identification of Bordetella from patients specimens |
| |
Authors: | S Cohen-Bacrie F Bertin A-S Gassiot M-F Prère |
| |
Institution: | 1. Unité de bactériologie pédiatrique, IFB, CHU de Toulouse, France;2. LMGM, IBCG, CNRS, université Paul-Sabatier, 118, route de Narbonne, 31062 Toulouse cedex 9, France |
| |
Abstract: | Biological diagnosis of whooping cough is increasingly necessary to confirm respiratory tract infection. Indeed, clinical symptoms are variable especially in adolescents and adults who contaminate newborns too young to be vaccinated. The PCR assay was proven highly sensitive for the diagnosis of pertussis. In this study, we reported the use of a new test (GenoQuick® Bordetella GQB], Hain Life Science, Germany) which permits the fast molecular genetic identification of Bordetella pertussis and parapertussis directly from patients specimens, i.e. swabs from nose or throat. The test was performed over a three months period on 40 specimens from patients (1 month to 65 years old), most of them were young children admitted in paediatric emergency with paroxysmal cough or prolonged cough. |
| |
Keywords: | Bordetella pertussis and parapertussis PCR GenoQuick® Bordetella test |
本文献已被 ScienceDirect 等数据库收录! |
|