| 用荧光染料fura-2法测定心室肌细胞内游离钙离子浓度 |
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| 引用本文： | 潘敬运,黄晓笛,詹澄扬.用荧光染料fura-2法测定心室肌细胞内游离钙离子浓度[J].中山大学学报(医学科学版),1990(2). |
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| 作者姓名： | 潘敬运 黄晓笛 詹澄扬 |
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| 作者单位： | 中山医科大学生理学教研室
(潘敬运，黄晓笛)，中山医科大学生理学教研室(詹澄扬) |
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| 摘 要： | 用fura-2荧光染料测定了静息时的酶解分离心室肌细胞内游离Ca~(2+)浓度,40mMKCI和5mM咖啡因都能使心室肌细胞内游离Ca~(2+)浓度增加,但KCI增加游离Ca~(2+)浓度需要细胞外液中存在Ca~(2+),而咖啡因则否,提示KCl增加细胞内游离Ca~(2+)浓度可能是通过细胞外Ca~(2+)内流,咖啡因则使细胞内贮存Ca~(2+)释放。
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| 关 键 词： | 心室肌细胞 细胞内游离Ca~(2+)浓度 KCl 咖啡因 |
MEASUREMENT OF THE INTRACELLULAR FREE CALCIUM CONCENTRATION OF VENTRICULAR MYOCYTES WITH FLUORESCENT DYE FURA-2 |
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| Pan Jingyun Huang Xiaodi Zhan Chengyang.MEASUREMENT OF THE INTRACELLULAR FREE CALCIUM CONCENTRATION OF VENTRICULAR MYOCYTES WITH FLUORESCENT DYE FURA-2[J].Journal of Sun Yatsen University(Medical Sciences),1990(2). |
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| Authors: | Pan Jingyun Huang Xiaodi Zhan Chengyang |
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| Institution: | Department of Physiology |
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| Abstract: | The intracellular free calcium concentration in the resting ventricle myocytes of rat was measured with fluorescent dye fura-2. Both 40mM KC1 and 5mM caffeine increased the intracellular Ca2+] of the ventricular myocytes. The rise in the intracellular Ca2+] by KC1 was dependent on the presence of the extracellular calcium, however, the rise in the intracellular Ca2+] by caffeine was not, suggesting that the increased Cytosolic Ca2+] by KC1 may be due to Ca2+ influx through voltage dependent calcium channels and caffeine may cause the release of calcium from intracellular calcium stores. |
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| Keywords: | Cardiac ventricular myocyte Intracellular free calcium concentration KC1 Caffeine |
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