运动训练对大鼠脑缺血再灌注后肢体功能恢复及微管相关蛋白含量的影响

目的　研究运动训练对局部脑缺血再灌注大鼠的肢体功能恢复是否具有促进作用 ,并同时从分子角度探讨其相关机制。方法　将大鼠随机分为运动组、对照组、假手术组及正常对照组 ,采用线栓法制作Wistar大鼠大脑中动脉闭塞 (middlecerebralarteryocclusion ,MCAO)再灌注模型。运动组大鼠术后每天进行游泳训练 ,采用前肢放置实验及平衡实验观察各组大鼠肢体功能恢复情况 ,并同时采用免疫组化法观察脑缺血周边区微管相关蛋白 2 (MAP 2 )的免疫活性强度。结果　大鼠局部脑缺血再灌注后 ,运动组大鼠前肢放置实验评分及平衡实验评分均优于对照组 ,运动组大鼠前肢放置实验评分在术后第 9,11,13 ,15,17,19,2 1,2 3及 2 5天时 ,较对照组差异均有极显著性意义 (P均 <0 .0 1) ;平衡实验评分在第 7,9,11,13 ,17及 19天时较对照组差异均有显著性意义 (P <0 .0 1或 0 .0 5) ;运动组大鼠皮层缺血周边区MAP 2表达水平亦高于对照组 ,在术后第 14 ,2 8天时较对照组差异具有显著性意义 (P均 <0 .0 5)。结论　运动训练能促进缺血再灌注大鼠的肢体功能恢复 ,其相关机制可能与上调缺血周边区MAP 2的表达有关

Effects of exercise training on the functional recovery of extremities and the concentration of microtubule associated protein 2 in rats with focal cerebral ischemia-reperfusion

Objective To study if exercise training ca n promote the functional recovery of extremities in rats with focal cerebral is chemia-reperfusion, and explore the relevant mechanism with a molecular perspec tive. Methods Fifty-two rats were recruited in thi s study and randomly divided into 4 groups: an exercise group, a control group, a sham operation group and a healthy control group. The middle cerebral artery o cclusion-reperfusion (MCAO-R) model was established by use of Zea-Longa's met hod in rats. The rats of the exercise group were administered with swimming trai ning daily after the operation. The sensorimotor functional recovery was evaluat ed by behavioral test involving the forelimb movement of the rat and the balance test. Meanwhile, immunoreaction activity of microtubule associated protein 2 ( MAP 2) in the surrounding area of cerebral ischemia tissue in each group were me asured by use of immunohistochemical method. Results After focal cerebral ischemia-reperfusion in rats, the scores of behavioral t est and the balance test in the exercise group are higher than those in the cont rol group. Significant difference was revealed between the scores of the behavio ral test in two groups at 9, 11, 13, 15, 17, 19, 21, 23, 25 days after operation ( P <0.01), as well as those of the balance test in two groups at 7, 9, 11, 1 3, 17, 19 days ( P <0.01). Concentration of MAP 2 in surrounding area of ische mic cerebral tissue in the exercise group was higher than that in the control gr oup, significant difference was revealed at 14, 28 days after operations ( P <0.05). Conclusion Exercise training may improve the functional recovery of extremities in rats with cerebral ischemia-reperfusi on, and the mechanism was related to the upregulative expression of MAP-2 in the surrounding area of the ischemic brain tissue.