| 噬菌体技术筛选广谱炎症相关趋化因子抑制剂先导物 |
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| 引用本文： | 李琴,孙晗笑,李秀英,莫雪梅,张光. 噬菌体技术筛选广谱炎症相关趋化因子抑制剂先导物[J]. 免疫学杂志, 2010, 26(6) |
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| 作者姓名： | 李琴 孙晗笑 李秀英 莫雪梅 张光 |
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| 作者单位： | 暨南大学药学院基因药物研究所,广州,510632;暨南大学药学院基因药物研究所,广州,510632;暨南大学药学院基因药物研究所,广州,510632;暨南大学药学院基因药物研究所,广州,510632;暨南大学药学院基因药物研究所,广州,510632 |
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| 基金项目： | 国家自然科学基金项目(30872221);;广州市重大科技计划(2005Z1-E4081);;广东省关键领域重大项目(粤科计字[2005]162号) |
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| 摘 要： | 目的:筛选能与炎症相关趋化因子结合的广谱抑制剂先导物。方法:用内毒素刺激外周血单核细胞建立急性炎症模型,以刺激炎症相关趋化因子的产生,利用噬菌体随机12肽库进行高通量筛选得到阳性克隆,并初步分析其体外活性。结果:经过四轮肽库亲和筛选后,获得了一共同短肽(TVVGSGCVLRIQ)。结论:该短肽可有效抑制炎性趋化因子活性,说明该短肽有可能成为一种广谱的炎性趋化因子抑制先导物。
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| 关 键 词： | 内毒素 噬菌体肽库 PBMC LUMINEX液相芯片 |
Screening broad-spectrum inflammatory-correlated chemokine inhibitor leader from phage random peptide library |
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| LI Qin,SUN Hanxiao,LI Xiuying,MO Xuemei,ZHANG Guang Institute of Genomic Medicine,College of Pharmacy,Jinan University,Guangzhou ,China. Screening broad-spectrum inflammatory-correlated chemokine inhibitor leader from phage random peptide library[J]. Immunological Journal, 2010, 26(6) |
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| Authors: | LI Qin SUN Hanxiao LI Xiuying MO Xuemei ZHANG Guang Institute of Genomic Medicine College of Pharmacy Jinan University Guangzhou China |
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| Affiliation: | LI Qin,SUN Hanxiao,LI Xiuying,MO Xuemei,ZHANG Guang Institute of Genomic Medicine,College of Pharmacy,Jinan University,Guangzhou 510632,China |
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| Abstract: | The aim of the study is to screen the broad-spectrum inflammatory chemokines inhibitor leader. We stimulated peripheral blood mononuclear cells (PBMC) with LPS to set up an acute inflammation model and to produce the inflammatory chemokines. Then by using the phage random peptide library, the positive clones were screened and their in vitro activity was analyzed. At last we found a common short peptide (TVVGSGCVLRIQ) by four screening rounds; the short peptide can effectively inhibit the inflammatory chemok... |
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| Keywords: | LPS Phage random peptide library Peripheral blood mononuclear cell LUMINEX liquid chip |
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