| 束缚应激反应对小鼠晶状体抗氧化能力指数的影响 |
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| 引用本文： | 江涛,赵亮,郑洁静,王璐璐,续洁琨,姚新生,栗原博.束缚应激反应对小鼠晶状体抗氧化能力指数的影响[J].中国药理学通报,2006,22(7):849-853. |
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| 作者姓名： | 江涛 赵亮 郑洁静 王璐璐 续洁琨 姚新生 栗原博 |
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| 作者单位： | 1. 暨南大学中药及天然药物研究所,广东,广州,510632
2. 沈阳药科大学中药学院,辽宁,沈阳,110016 |
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| 摘 要： | 目的探讨束缚应激反应对小鼠晶状体抗氧化能力指数的影响。方法建立小鼠束缚应激模型,采用荧光酶标仪测定晶状体的抗氧化能力指数,用高效液相-电化学检测器(HPLC-ECD)测定小鼠晶状体中谷胱甘肽含量。结果随着应激时间的延长,小鼠晶状体的抗氧化能力指数和谷胱甘肽含量与正常组相比均有不同程度下降。结论束缚应激状态下,晶状体的抗氧化能力指数和谷胱甘肽水平均相应降低,其机制可能与晶状体内的内源性抗氧化能力降低及氧自由基产生过多和蓄积有关。
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| 关 键 词： | 束缚应激 晶状体 抗氧化能力指数 谷胱甘肽 |
| 文章编号： | 1001-1978(2006)07-0849-05 |
| 收稿时间： | 02 9 2006 12:00AM |
| 修稿时间： | 04 16 2006 12:00AM |
Effects of restraint stress on oxygen radical absorbance capacity in murine lens |
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| JIANG Tao,ZHAO Liang,ZHENG Jie-jing,WANG Lu-lu,XU Jie-kun,YAO Xin-sheng,KURIHARA Hiroshi.Effects of restraint stress on oxygen radical absorbance capacity in murine lens[J].Chinese Pharmacological Bulletin,2006,22(7):849-853. |
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| Authors: | JIANG Tao ZHAO Liang ZHENG Jie-jing WANG Lu-lu XU Jie-kun YAO Xin-sheng KURIHARA Hiroshi |
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| Institution: | 1. Institute of Traditional Chinese Medicine and Natural Products, JiNan University, Guangzhou 510632, China, 2. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China |
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| Abstract: | Aim To investigate the effects of restraint stress on oxygen radical absorbance capacity(ORAC)in lens of mice.Methods Mice were loaded with restraint stress by being fixed in the restraint cage. In addition,ORAC activity in lens was determined with automated multifunctional microplate reader.The content of glutathione(GSH) in lens was determined with high performance liquid chromatography-electrochemical detector(HPLC-ECD).Results Restraint stress decreased the ORAC activity and GSH content of the lens compared with the normal mice in a time-dependent manner.Conclusion Restraint stress could decrease the ORAC activity and GSH level of the lens,which might be related to the decrease of free radical scavenging activity and the increase of oxygen radicals in the lens of mice loaded with restraint stress. |
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| Keywords: | restraint stress lens oxygen radical absorbance capacity(ORAC) glutathione(GSH) |
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