| Magnetic Iron Oxide Nanoparticles Mediated Gene Therapy for Breast Cancer-An In Vitro Study |
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| 作者姓名： | 韦卫中 徐春芳 吴华 |
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| 作者单位： | Wuhan Medical and Health Care center for Woman and Children Tongji Medical College Huazhong University of Science and Technology，Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Nuclear Medicine The First Hospital of Xiamen Fujian Medical University，Wuhan 430016 China，Wuhan 430030 China，Xiamen 361003 |
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| 基金项目： | This project was supported by a grant from the National Natural Science Foundation of China (No. 30171062). |
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| 摘 要： | Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), also designated as Apo-2 ligand, is a typical member of the structurally related tumor necrosis factor (TNF) ligand family. It has been shown that TRAIL only induces the apoptosis of cancer cells rather than normal cells. This characteristic offers a new target for cancer treatment1]. In fact, TRAIL gene has been successfully used for the treatment of cancer2]. Viruses have been the common vectors used in TRAIL gene t…
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| 关 键 词： | 磁铁 乳酸 基因治疗 乳腺癌 |
| 收稿时间： | 2006-09-20 |
| 修稿时间： | 2006-09-20 |
Magnetic iron oxide nanoparticles mediated gene therapy for breast cancer — An In Vitro study |
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| Weizhong Wei M.D., Ph.D.,Chunfang Xu,Hua Wu.Magnetic Iron Oxide Nanoparticles Mediated Gene Therapy for Breast Cancer-An In Vitro Study[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2006,26(6):728-730. |
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| Authors: | Weizhong Wei MD PhD Chunfang Xu Hua Wu |
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| Institution: | (1) Wuhan Medical and Health Care center for Woman and Children, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430016, China;(2) Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China;(3) Department of Nuclear Medicine, The First Hospital of Xiamen, Fujian Medical University, Xiamen, 361003, China |
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| Abstract: | Summary The aim of this study was to evaluate the feasibility and efficacy of using TRAIL gene to treat breast cancer mediated with
a novel carrier — magnetic iron oxide nanoparticles (polyMAG-1000) coated with PEI. The magnetic iron oxide nanoparticles
were used as gene carrier to transfect TRAIL gene into MCF-7 cells. The polyMAG-1000 without TRAIL gene was transfected into
the tumor cells as negative control. TRAIL gene transfection with liposome as carrier served as positive control. The apoptosis
of cells was detected with TUNEL method. The apoptosis ratio of tumor cells was measured with flow cytometry (FCM). It was
found that the apoptosis occurred in the tumor cells after transfection of TRAIL gene mediated by both polyMAG-1000 and liposome.
The apoptosis ratio in the group with polyMAG-1000 as gene carrier was (25.11 ± 2.85) %, whereas it was (5.06 ± 1.05) % in
the control group with polyMAG-1000 (P<0.01). The apoptosis ratio was as low as (18.31 ± 2.44) % in the group with liposome as gene carrier (P<0.05, as compared with the group with polyMAG-1000 as gene carrier). It is suggested that TRAIL gene may induce apoptosis
in MCF-7 breast cancer cells. The magnetic iron oxide nanoparticles coated with PEI may be a potential gene carrier with high
transfection efficacy for cancer gene therapy.
This project was supported by a grant from the National Natural Science Foundation of China (No. 30171062). |
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| Keywords: | Nanoparticles TRAIL gene therapy breast cancer |
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