不同用药途径促红细胞生成素用于治疗大鼠急性脊髓损伤的研究

目的：观察经不同给药途径给予重组人红细胞生成素(EPO)治疗脊髓损伤后大鼠神经功能行为，caspase-3表达以及凋亡的变化，探讨其脊髓保护的作用机制。方法：30只SD大鼠随机分成假手术组、对照组、蛛网膜下腔给药组(治疗A组)，尾静脉给药组(B组)，腹腔给药组(C组)，每组6只。观察药物治疗前后大鼠的神经功能行为变化，应用HE染色观察神经元病理变化，免疫组化染色检测caspase-3表达，TUNEL法标记凋亡细胞；比较各组间差异。结果：HE，免疫组化染色示各EPO治疗组脊髓损伤程度小，神经元细胞破坏少；c

Effect of rh-EPO injected in different approaches on spinal cord injury in rats

Objective: To investigate the effect of rH-EPO on expression level of casepase-3 and apoptosis after spinal cord injury(SCI), and to explore its mechonism of neuroprotective effect and to discuss the difference of different approaches. Method: Thirty SD rats were divided into five groups by randomization: sham group, control group, rh-EPO injected in to subarachnoid space group(A), injected in to tail vein group(B) and injected in to abdominal cavity group(C). There were 6 rats in each group. The changes of nerve function were observed. The expression of caspase-3 was detected with immunohistochemistry and the apoptosis was labeled by TUNEL. The differences and effects of each group were compared. Result: In EPO cure group, HE staining pathological changes were mitigated than that in control group. The caspase-3 protein level was lower than that in control group. Tunel-positive cells decreased significantly at the 14th d; the grade of nerve function improved distinctly. The effect of rh-EPO injected in to subarachnoid space group was better than that in other groups. There was no difference between group B and group C. Conclusion: EPO can inhibit the expression of caspase-3 and lessen neuronal and glial cell apoptoses in the rats after SCI, which may play a significant neuroprotective role in the secondary SCI. The effect of rh-EPO injected in to subarachnoid space group was the best.