Pentraxin-3 levels in gingival crevicular fluid during orthodontic tooth movement in young and adult patients

Abstract Objectives: To measure the levels of pentraxin-3 (PTX-3) in gingival crevicular fluid (GCF) in orthodontic young and adult patients in the first 2?weeks after the orthodontic appliance to determine whether those changes occur during orthodontic treatment and if those values could be the expression of an inflammatory state. Materials and Methods: GCF samples were collected with paper strips from 16 orthodontic young patients and 13 orthodontic adult patients from an upper canine requiring distalization as a test tooth. A contralateral canine was used as a control tooth. The absorbed volume was eluted in 100?μL phosphate-buffered saline (pH = 7.2). PTX-3 levels in GCF were determined using a commercial enzyme-linked immunosorbent assay kit, and the results were expressed in ng/mL. Results: The results showed an increase of GCF levels of PTX-3 from 1?hour before the orthodontic appliance to a maximum at 24?hours, followed by a decrease in both groups of adult and young patients. Conclusions: The results suggest PTX-3 involvement in periodontal orthodontic remodeling and the aseptic inflammation induced by the orthodontic forces.