白蔹、乌头合用对小鼠心、肝、肾功能及病理形态的影响

目的:观察十八反药物白蔹反乌头应用时对小鼠的毒性作用。方法:连续14 d给予小鼠乌头、白蔹、乌头-白蔹水煎剂,末次给药后30 min取小鼠血液并分离血清,测定血清总蛋白(TP)、血清白蛋白(ALB)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、尿素氮(BUN)、肌酐(Cr)、乳酸脱氢酶(LDH);处死后进行尸检,取心、肝、肾测定脏器系数并做病理检查。结果:血液生化指标检查结果表明,单用乌头组ALT,AST值升高(P<0.05);单用白蔹组AST升高(P<0.05);两药合用组ALT,AST,LDH均升高(P<0.01或P<0.05);其余各组各项生化指标的变化均在正常值范围内波动。脏器系数检查结果表明:单用乌头组肝脏系数增加(P<0.05),两药合用组肝、肾脏器系数均增加(P<0.01或P<0.05)。尸检及病理组织学检查结果表明:两药合用组小鼠肝、肾可见明显改变。结论:十八反药物乌头与白蔹合用时对小鼠毒性增强。

Influence of the Joint Medication of Ampelopsis japonica Root and Aconitum carmichaelii Root on the Function and Pathological Morphology of the Heart, Liver and Kidneyin Rats

Objective:To observe theinfluence of the joint medication of Ampelopsis japonica root and Aconitum carmichaelii root on the function and pathological morphology of the heart, liver and kidney in rats. Method: Rats were administrated with water decoction of A. japonica and A. carmichaelii for successive 14 days. At thirty minutes after the last administration, the serum levels of total serum protein(TP), alburnin(Alb), alanine aminotransferase(ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine (Cr)and lactate dehydrogenase (LDH) were measured. Autopsy was conducted after the death of the rats and the heart, liver, kidney were harvested for pathological examination.The index of viscera was calculated. Result: The levelof ALT and AST (P<0.05)was increased in the A. japonica group; the level of AST (P<0.05) was increased in the aconitum group. When treated with A. japonica and A. carmichaelii jointly, the level of ALT,AST and LDH (P<0.01 or P<0.05)all increased. The examination of organ index showed that the liver index increased(P<0.05) in A. japonica groupo and all the indexes (P<0.01 or P<0.05)of liver and kidney increased when A. japonica and aconitum A. carmichaelii were administrated jointly. Autopsy and histopathological examination indicated that the liver and kidney of the rats changed significantly when A. japonica and A. carmichaelii were administrated jointly. Conclusion: The toxicity of the joint medication of A. carmichaelii japonica and A. carmichaelii in rats would be strengthened.