| 脱氢氯甲睾酮人体内代谢研究 |
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| 引用本文： | 刘春胜,张霁,周同惠. 脱氢氯甲睾酮人体内代谢研究[J]. 药学学报, 1991, 26(9): 682-687 |
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| 作者姓名： | 刘春胜 张霁 周同惠 |
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| 作者单位： | 中国医学科学院药物研究所分析室，中国医学科学院药物研究所分析室，中国医学科学院药物研究所分析室 北京 100050，北京 100050，北京 100050 |
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| 摘 要： | 本文叙述了用GC-MS联用技术研究脱氢氯甲睾酮人体内代谢的方法。尿样中的甾体化合物经大孔树脂吸附提取后,酶解。浓缩并衍生化,进行GC-MS分析。在服药后8～30h的尿样中,检出了脱氢氯甲睾酮原型,并发现了七个代谢产物。分析色谱和质谱数据,得到了这些代谢物的结构及其浓度变化趋势,推测了脱氢氯甲睾酮可能的体内代谢模式,确定了筛检尿中脱氢氯甲睾酮的特定代谢物和特征检测离子,比较了不同的样品处理方法对分析结果的影响。
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| 关 键 词： | 脱氢氯甲睾酮 体内代谢 GC-MS |
| 收稿时间： | 1990-06-22 |
INVESTIGATION ON THE METABOLISM OF DEHYDROCHLOROMETHYLTESTOSTERONE IN MAN |
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| CS Liu,J Zhang and TH Zhou. INVESTIGATION ON THE METABOLISM OF DEHYDROCHLOROMETHYLTESTOSTERONE IN MAN[J]. Acta pharmaceutica Sinica, 1991, 26(9): 682-687 |
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| Authors: | CS Liu J Zhang TH Zhou |
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| Affiliation: | Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing. |
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| Abstract: | A GC-MS method for the investigation on the metabolism of dehydrochloromethyltestosterone (DHCMT)in man is described. Steroids in human urine were adsorbed on a macroporous XAD - 2 resin, eluted with methanol, hydrolyzed with glucuronidase, extracted and concentrated for derivatization and then analyzed with GC-MS. DHCMT and its 7 metabolites were detected in urine samples 8 to 30 h after oral administration. Their structures and the variation of their concentration in urine were determined and the possible metabolic pathways of DHCMT were proposed on the basis of GC- MS feature of its metabolites. The specific metabolites and characteristic ions for screening DHCMT positive urine were chosen. Furthermore, the influence of the sample pretreatment methods on GC- MS results has been studied. |
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| Keywords: | Dehydrochloromethyltestosterone Metabolism GC- MS |
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