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脱氢氯甲睾酮人体内代谢研究
引用本文:刘春胜,张霁,周同惠. 脱氢氯甲睾酮人体内代谢研究[J]. 药学学报, 1991, 26(9): 682-687
作者姓名:刘春胜  张霁  周同惠
作者单位:中国医学科学院药物研究所分析室,中国医学科学院药物研究所分析室,中国医学科学院药物研究所分析室 北京 100050,北京 100050,北京 100050
摘    要:本文叙述了用GC-MS联用技术研究脱氢氯甲睾酮人体内代谢的方法。尿样中的甾体化合物经大孔树脂吸附提取后,酶解。浓缩并衍生化,进行GC-MS分析。在服药后8~30h的尿样中,检出了脱氢氯甲睾酮原型,并发现了七个代谢产物。分析色谱和质谱数据,得到了这些代谢物的结构及其浓度变化趋势,推测了脱氢氯甲睾酮可能的体内代谢模式,确定了筛检尿中脱氢氯甲睾酮的特定代谢物和特征检测离子,比较了不同的样品处理方法对分析结果的影响。

关 键 词:脱氢氯甲睾酮  体内代谢  GC-MS
收稿时间:1990-06-22

INVESTIGATION ON THE METABOLISM OF DEHYDROCHLOROMETHYLTESTOSTERONE IN MAN
CS Liu,J Zhang and TH Zhou. INVESTIGATION ON THE METABOLISM OF DEHYDROCHLOROMETHYLTESTOSTERONE IN MAN[J]. Acta pharmaceutica Sinica, 1991, 26(9): 682-687
Authors:CS Liu  J Zhang  TH Zhou
Affiliation:Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Abstract:A GC-MS method for the investigation on the metabolism of dehydrochloromethyltestosterone (DHCMT)in man is described. Steroids in human urine were adsorbed on a macroporous XAD - 2 resin, eluted with methanol, hydrolyzed with glucuronidase, extracted and concentrated for derivatization and then analyzed with GC-MS. DHCMT and its 7 metabolites were detected in urine samples 8 to 30 h after oral administration. Their structures and the variation of their concentration in urine were determined and the possible metabolic pathways of DHCMT were proposed on the basis of GC- MS feature of its metabolites. The specific metabolites and characteristic ions for screening DHCMT positive urine were chosen. Furthermore, the influence of the sample pretreatment methods on GC- MS results has been studied.
Keywords:Dehydrochloromethyltestosterone  Metabolism  GC- MS
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