| 载体介导的RNAi技术抑制宫颈癌CaSki细胞E7基因的表达 |
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| 引用本文： | 王丹青,彭芝兰,牛晓宇,李大可.载体介导的RNAi技术抑制宫颈癌CaSki细胞E7基因的表达[J].四川大学学报(医学版),2007,38(1):6-8,48. |
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| 作者姓名： | 王丹青 彭芝兰 牛晓宇 李大可 |
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| 作者单位： | 四川大学华西第二医院,妇产科,成都,610041 |
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| 摘 要： | 目的 探讨HPV16E7特异性小干扰RNA(siRNA)表达载体对宫颈癌CaSki细胞E7基因的抑制作用.方法 构建HPV16 E7特异性siRNA表达载体,利用脂质体转染CaSki细胞,以荧光定量RT-PCR及Western blot检测其对E7 mRNA及蛋白表达的影响.结果 3种表达载体P1、P2、P3均能抑制CaSki细胞E7基因mRNA和蛋白的表达,其中载体P1抑制作用最强,在转染后3周,对E7 mRNA和蛋白的抑制率分别为92.86%和81.0%.结论 HPV16E7 siRNA表达载体能有效地抑制宫颈癌CaSki细胞E7基因的表达.
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| 关 键 词： | HPV16E7 小干扰RNA表达载体 宫颈癌 CaSki细胞 RNA干扰 载体介导 RNAi 宫颈癌 细胞 基因 蛋白表达 Technique RNA Interference Cervical Cancer Cells Oncogene 体能 抑制率 脂质体转染 结果 影响 mRNA 检测 Western blot |
| 收稿时间： | 2006-06-02 |
| 修稿时间： | 2006-09-18 |
Inhibiting E7 Oncogene in CaSki Cells of Cervical Cancer by Vector-based RNA Interference Technique |
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| WANG Dan-qing,PENG Zhi-lan,NIU Xiao-yu,LI Da-ke.Inhibiting E7 Oncogene in CaSki Cells of Cervical Cancer by Vector-based RNA Interference Technique[J].Journal of West China University of Medical Sciences,2007,38(1):6-8,48. |
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| Authors: | WANG Dan-qing PENG Zhi-lan NIU Xiao-yu LI Da-ke |
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| Institution: | Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China. |
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| Abstract: | Objective To test of the effect of vector-based RNA interference(RNAi) technique on inhibiting HPV16E7 gene in CaSki cells of cervical cancer.Methods The HPV16E7-specific siRNA expression vectors P1,P2 and P3 were constructed and transfected into CaSki cells by liposome.The expression of HPV16E7 mRNA and protein were detected by real-time RT-PCR and Western blot.Results The expression of HPV16E7 mRNA and protein decreased with the transfection of P1,P2 and P3.Vector P1 had the strongest inhibition effect,with an inhibition rates of 92.86% and 81.0% for the expression of HPV16E7 mRNA and protein respectively three weeks after transfection.Conclusion The expression of E7 gene in CaSki cells can be inhibited by HPV16E7-specific siRNA expression vector. |
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| Keywords: | HPV16E7 siRNA expression vector Cervical cancer CaSki ceils RNAi |
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