藿香正气液对湿阻证大鼠结肠黏膜水通道蛋白4表达的影响

目的:研究藿香正气液对湿阻证大鼠结肠黏膜水通道蛋白4(AQP4)表达的影响.方法:将24只SD大鼠随机分为正常组、模型组、藿香正气液组,每组8只.正常组常规喂养,其余各组采用改进的环境加疲劳法制造湿阻证模型,连续造模6d.造模成功后,正常组和模型组予生理盐水ig(20 mL·kg-1),治疗组予藿香正气液ig (20 mL·kg-1),1次/d,连续8d.分别采用免疫组化和Real-Time PCR的方法观察结肠黏膜AQP4的表达情况.结果:与正常组相比,模型组大鼠脾虚症状明显,胃黏膜有明显充血水肿,并有出血点及血块,大鼠结肠黏膜AQP4表达明显减少(P＜0.05);而经藿香正气液治疗后,与模型组比较,AQP4表达量有所增加(P＜0.05).结论:藿香正气液可明显改善湿阻证大鼠脾虚腹泻等症状,其机制可能与提高结肠黏膜AQP4的表达量有关.

Effects of Huoxiang Zhengqi Liquid on the Expression of AQP4 in Colon Mucosa in Rats with Dampness Retention Syndrome

Objective:To investigate the effects of Huoxiang Zhengqi liquid on the expression of AQP4 in colon mucosa in rats with dampness retention syndrome (DRS). Method:Twenty four SD rats were randomly divided into 3 groups:control group,DRS group and treatment groups (DRS+Huoxiang Zhengqi liquid), 8 rats in each group.DRS was induced in all rats,except the control group,by modified environmental and fatigued method for 6 days.After DRS model was copied,control and DRS groups were orally given with normal saline(NS, 20 mL·kg^-1). Rats in treatment groups were orally given with Huoxiang Zhengqi liquid(20 mL·kg^-1) for 8 days.Then the expression of aquaporin 4(AQP4) on colon mucosa was determined by immunohistochemistry and Real-Time PCR. Result:Rats in model groups exhibited typical DRS with sign of hyperaemia, edema, hemorrhagic spots and bleeding in the gastric mucosa compared to control. The expression of AQP4 in colon musoca significantly decreased in DRS group compared to control group(P<0.05).Conversely, exposure of DRS rats to Huoxiang Zhengqi liquid resulted in increase in expression in AQP4 in the colon mucosa(P<0.05 versus DRS group). Conclusion:Huoxiang Zhengqi liquid could have the effect of curing the DRS-induced diarrhoea, increased expression of AQP4 may play an important role.