高效液相色谱法测定安宫牛黄丸中栀子苷、黄芩苷和盐酸小檗碱的含量

目的：建立同时测定安宫牛黄丸中栀子苷、黄芩苷和盐酸小檗碱含量的反相液相色谱法。方法：采用Agilent ZORBAX SB-C₁₈柱(4.6 mm×250 mm,5 μm),乙腈-6 mmol·L^-1 KH₂PO₄水溶液(pH 4.6)梯度洗脱,检测波长240 nm。结果：栀子苷、黄芩苷和盐酸小檗碱的线性范围分别为4.50～110.00,5.00～153.00,6.40～191.00 mg·L^-1；质量检测下限分别为0.77,1.53,1.43 ng；栀子苷的回收率为104.44%,RSD为1.79%；黄芩苷的回收率为96.98%,RSD为1.76%；盐酸小檗碱的回收率为101.08%,RSD为3.1%。结论：采用该方法同时测定安宫牛黄丸中栀子苷、黄芩苷和盐酸小檗碱含量具有简便﹑快速﹑准确等优点。

Simultaneous determination of geniposide,baicalin and berberine hydrochloride in Angong Niuhuang pill by RP-HPLC

Objective: A new method for the simultaneous quantitative determination of geniposide, baicalin and berberine hydrochloride in Angong Niuhuang pill using reversed phase high performance liquid chromatographic method had been developed. Method: The optimum chromatographic conditions were as follows: Agilent Zorbax SB-C₁₈ column (4.6 mm 250 mm, 5 m), acetonitrile-H₂O (containing 6 mmol·L^-1 KH₂PO₄, pH 4.6) gradient elution; as a detection wavelength of 343 nm. Result: The calibration curves of geniposide, baicalin and berberine were linear at the ranges of 4.50-110.00,5.00-153.00, 6.40-191.00 mg·L^-1, respectively. The limits of detection of the method were 0.77 ng for geniposide, 1.53 ng for baicalin and 1.43 ng for berberine hydrochloride. The recoveries of the method were 104.44% (RSD 1.79%) for geniposide, 96.98% (RSD 1.76%) for baicalin, 101.08% (RSD 3.1%) for berberine hydrochloride. Conclusion: This method had been successfully applied to determine the content of geniposide, baicalin and berberine hydrochloride in Angong Niuhuang pill.