Reciprocal stimulation of gammadelta T cells and dendritic cells during the anti-mycobacterial immune response |
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Authors: | Dieli Francesco Caccamo Nadia Meraviglia Serena Ivanyi Juraj Sireci Guido Bonanno Cesira T Ferlazzo Viviana La Mendola Carmela Salerno Alfredo |
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Affiliation: | Dipartimento di Biopatologia, Università di Palermo, Palermo, Italy. dieli@unipa.it |
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Abstract: | Gammadelta T cells and dendritic cells (DC) are two distinct cell types of innate immunity that participate in early phases of immune response against Mycobacterium tuberculosis infection. Here we show that a close functional relationship exists between these cell populations. Using an in vitro coculture system, Vgamma1 T cells from Tcrb(-/- )mice were found to be activated by DC infected in vitro with BCG, as indicated by the elevated CD69 expression, IFN-gamma secretion and cytotoxic activity. This activation process was due to a non-cognate mechanism since it required neither cell to cell contact nor interaction between the TCR and a specific antigen, but was mediated by DC-derived IL-12. Reciprocally, Vgamma1 T cells provided a key cytokine, IFN-gamma, which increased IL-12 production by BCG-infected DC. Moreover, exposure of BCG-infected DC to Vgamma1 T cells conditioned the former to prime a significantly stronger anti-mycobacterial CD8 T cell response. Consequently, stimulation of gammadelta T cells and their non-cognate interaction with DC could be applied as an immune adjuvant strategy to optimize vaccine-induced CD8 T cell immunity. |
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Keywords: | γδ T cells Dendritic cells Mycobacterium bovis Bacillus Calmette‐Guerin Cytokines |
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