亚低温下缺血预处理对肝缺血再灌注损伤的影响

目的：探讨亚低温下缺血预处理（MHIP）对肝缺血再灌注后超微结构的影响。方法：将24只犬随机分为4组：非缺血对照组、缺血再灌注组、缺血预处理组和亚低温血预处理组，用透射电镜观察各组肝组织超微结构改变。结果：肝缺血再灌注组超微结构损伤较重；缺血预处理组和亚低温缺血预处理线超微结构改变的较小，但亚低温缺血预处理组与缺血预处理组比较线粒体保护更好（P＜0．01，P＜0．05）。结论：缺血预处理对肝缺血再灌注后超微结构具有保护作用，亚低浊缺血预处理的这种保护作用具有增强效应。

Influence of mild hypothermic ischemic preconditioning on liver ultrastructure damage followed ischemia-reperfusion injury

Objective To study the influence of mild hypothermic ischemic preconditioning on liver ultrastructure followed ischemia -reperfusion injury.Methods 24 dogs were randomly divided into four groups: nonischemic controls(C) group( n =6), ischemia-reperfusion (I-R) group ( n =6),ischemic preconditioning(IP) group( n =6) and mild hypothermic ischemic preconditioning(MHIP) groups( n =6). Ultrastructual changes of liver were studied by electron microscope. Results Ultrastructural damage of the liver was more severe in I-R; Ultrastructual changes of liver was mild in both of IP and MHIP,but protective effects to mitochondria was better in MHIP than that in IP( P<0.01, P<0 .05). Conclusion Ischemic preconditioning has protective effects on ultrastructural damage of the liver after ischemia- reperfusion;Mild hypothermia can enhance the protective effect of IP followed hepatic ischemia -reperfusion injury.