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Tityus serrulatus venom peptidomics: Assessing venom peptide diversity |
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| Authors: | Breno Rates,Karla K.F. Ferraz,Má rcia H. Borges,Maria Elena De Lima,Adriano M.C. Pimenta |
| Affiliation: | a Laboratório de Venenos e Toxinas Animais, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, Bloco O4, Sala 125, 6627, 31270-901, Belo Horizonte, Minas Gerais, Brasil b Núcleo de Estudo de Estrutura e Função de Biomoléculas, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31270-901, Belo Horizonte, Minas Gerais, Brasil c Centro de Pesquisa Professor Carlos R. Diniz, Fundação Ezequiel Dias, Rua Conde Pereira Carneiro 80, 30550-010, Belo Horizonte, MG, Brasil |
| Abstract: | MALDI-TOF-TOF and de novo sequencing were employed to assess the Tityus serrulatus venom peptide diversity. Previous works has shown the cornucopia of molecular masses, ranging from 800 to 3000 Da, present in the venom from this and other scorpions species. This work reports the identification/sequencing of several of these peptides. The majority of the peptides found were fragments of larger venom toxins. For instance, 28 peptides could be identified as fragments from Pape proteins, 10 peptides corresponded to N-terminal fragments of the TsKβ (scorpine-like) toxin and fragments of potassium channel toxins (other than the k-beta) were sequenced as well. N-terminal fragments from the T. serrulatus hypotensins-I and II and a novel hypotensin-like peptide could also be found. This work also reports the sequencing of novel peptides without sequence similarities to other known molecules. |
| Keywords: | Tityus serrulatus Venom MALDI-TOF-TOF De novo sequencing |
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