梅罗综合征病理分型的免疫组织化学和电镜研究

目的：进一步实梅罗综合征的病理分型的并观察其肉芽肿瘤细胞的形态特征。方法：收集２０例，用抗溶菌酶抗体免疫组织化学的方法及透射电镜对其进行标记和观察。结果：肉芽肿型可见阳性细胞灶，电镜下有多核巨细胞、类上皮细胞等肉芽肿细胞，且有超微特征。而非肉牙肿型为阴性结果。结论：进一步证实了梅罗综合征有肉芽肿型和非肉芽肿型两型病变，并认为前者肉芽肿泖而小是其区别结节病的结核病等的形态特征之一。

The Immunohistochemical and Electron Microscopical Study of Pathological Classification of Melkersson-Rosenthal Syndrome

OBJECTIVE: To prove further the pathological classification of Melkersson-Rosenthal syndrome (MRS) and observe the morphological features of the granuloma cells in MRS. METHODS: We studied 20 cases of MRS, with 10 cases of granuloma type and the other 10 cases of non-granuloma type. The negative results of periodic acid-schiff (PAS), Neelren anti-acid special staining and tuberculosis (TB)-DNA polymerase chain reaction proved that all cases were not TB and fungous disease. Then all the cases were labelled with antibody of lysozyme by immunohistochemical staining and investigated with electron microscope. We studied the expression of lysozyme in multinucleated giant cells, epithelioid cells and histiocytes. RESULTS: The results of lysozyme staining were positive in the multinucleated giant cells, epithelioid cells and histiocytes of the granuloma type and were negative in those cells of the non-granuloma type. On the other hand, the small multinucleated giant cells which had 2-3 nuclei were the features of the morphological structure of the granuloma type. The small multinucleated giant cells were not seen in the non-granuloma type. CONCLUSION: In this paper, two pathological types (granuloma and non-granuloma type) in the MRS were proved further and it is found that the different structures of granuloma can be used to discriminate between MRS and sarcoidosis and tuberculosis.